J Korean Bone Joint Tumor Soc.  2014 Jun;20(1):1-6. 10.5292/jkbjts.2014.20.1.1.

The Role of CTGF in Osteosarcoma Progression

• Han I
• Lee MR
• Kim HS

• KMID: 1707758
• DOI: http://doi.org/10.5292/jkbjts.2014.20.1.1

Abstract

PURPOSE
To examine the expression of Connective Tissue Growth Factor (CTGF) in osteosarcoma and to evaluate its role in osteosarcoma invasion and proliferation.
MATERIALS AND METHODS
The mRNA expression of CTGF from 23 patient-derived osteosarcoma cell lines was examined, and the role of CTGF in cell invasion and proliferation was examined using siRNA transfection.
RESULTS
The over-expression of CTGF mRNA was observed in 17 cell lines (74%). CTGF-specific siRNA transfection into SaOS-2 and MG63 cell lines resulted in efficient knockdown of CTGF expression on Western blot analysis. siRNA transfected cells showed decreased migration on Matrigel invasion assay and decreased cell proliferation on WST-1 assay.
CONCLUSION
These results indicated that the CTGF expression may play an important role in osteosarcoma progression, and may be a therapeutic target of osteosarcoma.

Keyword

osteosarcoma; connective tissue growth factor; CCN; CTGF; cancer progression

MeSH Terms

Blotting, Western
Cell Line
Cell Proliferation
Connective Tissue Growth Factor
Osteosarcoma*
RNA, Messenger
RNA, Small Interfering
Transfection
Connective Tissue Growth Factor
RNA, Messenger
RNA, Small Interfering

Figure

• 

  Figure 1. mRNA Expression of CTGF in 23 patient-derived osteosarcoma cell lines. Human osteoblast cell line (hFOb 1.19) was used as the control cell line.

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  Figure 2. siRNA knockdown of CTGF in osteosarcoma cell lines. (A, B) Western blot analysis and (C, D) Real-time PCR. MG63 (A, C) and SaOS-2 (B, D) cell lines were used. *p<0.05.

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  Figure 3. Representative microscopic image (×100) of Matrigel invasion assay. The number in the CTGF-siRNA transfected cells (B) is reduced compared to the control cells (A).

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  Figure 4. Effect of CTGF in osteosarcoma proliferation. The number of viable cells is reduced in the CTGF-siRNA transfected cells. MG63 (A) and SaOS-2 (B) cell lines were used. *p<0.05.

Reference

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