J Korean Acad Nurs.  2013 Jun;43(3):361-369. 10.4040/jkan.2013.43.3.361.

Effect of Anorexia and Neuropathic Pain Induced by Cisplatin on Hindlimb Muscles of Rat

• Yang GS
• Choe MA

• KMID: 1707126
• DOI: http://doi.org/10.4040/jkan.2013.43.3.361

Abstract

PURPOSE
The purpose of this study was to examine the effect of anorexia and neuropathic pain induced by cisplatin on hindlimb muscles of rats.
METHODS
Adult male Sprague-Dawley rats were divided into two groups, a cisplatin-treated group (n=10) and a control group (n=10). In the cisplatin-treated group, cisplatin at a dose of 2 mg/kg was injected intraperitoneally two times a week up to a cumulative dose of 20 mg/kg over 5 weeks, and in the control group saline (0.9% NaCl) was injected intraperitoneally at the same dose and duration as the cisplatin-treated group. At 34 days all rats were anesthetized, after which the soleus and plantaris muscles were dissected. Withdrawal threshold, body weight, food intake, activity, muscle weight, Type I and II fiber cross-sectional areas and myofibrillar protein content of the dissected muscles were determined.
RESULTS
Compared with the control group, the cisplatin-treated group showed significant decreases (p<.05) in withdrawal threshold, activity, food intake, body weight, Type I and II fiber cross-sectional areas, myofibrillar protein content and weight of the soleus and plantaris muscles.
CONCLUSION
Muscular atrophy in hindlimb occurs due to anorexia and neuropathic pain induced by the cisplatin treatment.

Keyword

Cisplatin; Neuropathic pain; Anorexia; Muscular atrophy

MeSH Terms

Animals
*Anorexia
Body Weight
Cisplatin/*toxicity
Eating
Hindlimb
Injections, Intraperitoneal
Male
Motor Activity
Muscle Fibers, Skeletal/metabolism/pathology
Muscle Proteins/metabolism
Muscle, Skeletal/*drug effects/physiology
Neuralgia/*chemically induced/pathology
Rats
Rats, Sprague-Dawley
Cisplatin
Muscle Proteins