J Vet Sci.  2013 Sep;14(3):241-248. 10.4142/jvs.2013.14.3.241.

GC/MS analysis of high-performance liquid chromatography fractions from Sophora flavescens and Torilis japonica extracts and their in vitro anti-neosporal effects on Neospora caninum

Affiliations
  • 1Pfizer Animal Health, Seoul 135-784, Korea.
  • 2College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. younhj@snu.ac.kr
  • 3College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea.

Abstract

We analyzed alcoholic extracts of herbs possessing anti-neosporal activity against Neospora (N.) caninum. To identify the chemical components of Sophora (S.) flavescens and Torilis (T.) japonica associated with anti-neosporal activity, specific fractions were isolated by high-performance liquid chromatography (HPLC). In vitro activity of the fractions against N. caninum was then assessed. Gas chromatography/mass spectrometry (GC/MS) was used to identify and quantify specific anti-neosporal molecules in the herbal extracts. Almost all HPLC fractions of S. flavescens and T. japonica had higher levels of anti-neosporal activity compared to the not treated control. Active constituents of the extracts were sophoridane, furosardonin A, and tetraisopropylidene-cyclobutane in S. flavescens; 5,17-beta-dihydroxy-de-A-estra-5,7,9,14-tetraene, furanodiene, and 9,12-octadecadienoic acid (Z,Z)-(CAS,1) in T. japonica.

Keyword

GC/MS; HPLC; Neospora caninum; Sophora flavescens; Torilis japonica

MeSH Terms

Apiaceae/*chemistry
Chromatography, High Pressure Liquid
Coccidiostats/*chemistry
Fruit/chemistry
Gas Chromatography-Mass Spectrometry
Neospora/*drug effects/growth & development
Plant Extracts/*chemistry
Plant Roots/chemistry
Sophora/*chemistry
Coccidiostats
Plant Extracts
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