Korean J Med.  2003 Aug;65(2):178-187.

The effects of ecabet sodium on nuclear factor-kappa B activation and chemokine gene expression in Helicobacter pylori-infected human gastric epithelial cells

Affiliations
  • 1Department of Microbiology and Institute of Biomedical Science, Hanyang University College of Medicine, Seoul, Korea.
  • 2Department of Internal Medicine and Liver Research Institute, College of Medicine, Seoul National University, Seoul, Korea. hyunchae@plaza.snu.ac.kr

Abstract

BACKGROUND: Helicobacter pylori stimulates nuclear factor-kappa B (NF-kappa B) activation and chemokine expression of gastric epithelial cells. Although ecabet sodium (ecabet), a locally acting anti-ulcer drug, is known to have an anti-H. pylori activity, there is little known how ecabet acts anti-inflammatory effects in gastric epithelial cells infected with H. pylori. We investigated the effects of ecabet on chemokine gene expression and NF-kappa B activation of human gastric epithelial cells infected with H. pylori.
METHODS
After the infection of Hs746T and MKN-45 gastric epithelial cell lines with cagA+cytotoxin+ H. pylori in the presence of ecabet, mRNA expression of chemokine such as interleukin-8 (IL-8) and monocyte chemotactic protein-1 (MCP-1) was assessed by quantitative RT-PCR, and chemokine secretion was measured by ELISA. NF-kappa B signals were assayed by electrophoretic mobility shift assay. The activation of NF-kappa B and IL-8 reporter genes was measured by luciferase assay.
RESULTS
The treatment of ecabet (5 microgram/mL) decreased the transcription and secretion of chemokine IL-8 and MCP-1 from the gastric epithelial cells infected with H. pylori in a dose-dependent manner. In addition, ecabet inhibited NF-kappa B activation of gastric epithelial cells induced by H. pylori infection. Moreover, the inhibited NF-kappa B signal by ecabet was comprised of heterodimers of p65/p50 predominantly.
CONCLUSION
These results suggest that ecabet can inhibit H. pylori-induced IL-8 and MCP-1 gene transcription via suppression of NF-kappa B signal.

Keyword

Ecabet sodium; Helicobacter pylori; Chemokine; NF-kappa B

MeSH Terms

Chemokine CCL2
Electrophoretic Mobility Shift Assay
Enzyme-Linked Immunosorbent Assay
Epithelial Cells*
Gene Expression*
Genes, Reporter
Helicobacter pylori
Helicobacter*
Humans*
Interleukin-8
Luciferases
NF-kappa B
RNA, Messenger
Sodium*
Chemokine CCL2
Interleukin-8
Luciferases
NF-kappa B
RNA, Messenger
Sodium
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