Korean J Nutr.  2003 May;36(4):382-388.

Induction of Apoptosis by (-)-epigallocatechin-3-gallate in HL-60 Cells

Affiliations
  • 1Department of Food and Nutrition, Yonsei University, Seoul, Korea.

Abstract

(-)-Epigallocatechin-3-gallate (EGCG) is a polyphenolic compound found in peen tea leaves, and has been known to be one of the most potent catechin species which inhibits cell growth most possibly through an apoptotic cell death. We investigated the apoptotic activity of (-)-EGCG on the human myeloid leukemia cell line, HL-60. Our results of MTT test indicated that (-)-EGCG had a significant antiproliferation effect in HL-60 cells with IC50 (50% inhibition concentration) value of 65 micrometer. Giemsa statining of HL-60 cells treated with (-)-EGCG (100 micrometer) for 6hrs showed a typical apoptosis-specific morphological change including shrinkage of the cytoplasm, membrane blobbing and compaction of the nuclear chromatin. The DNA fragmentation was observed from the agarose gel electrophoresis of cells treated with (-)-EGCG for 3hrs or longer, and was progressed to a greater degree as treatment time increases. Treatment of the cells with (-)-EGCG (100 micrometer) resulted in a rapid release of mitochondrial cytochrome c into the cytosol, and a subsequent cleavage of caspase-3 to an active form in a treatment-time dependent manner. (-)-EGCG (100 micrometer) also stimulated proteolytic cleavage of poly-(ADP-ribose) polymerase (PARP) to an active form in HL-60 cells. Tlken together, (-)-EGCG appears to induce the apoptosis in human myeloid leukemia cells via a caspase-dependent pathway. These results suggest the possible application of (-)-EGCG, the major active compound in green tea, as an antiproliferative agent for cancer prevention.

Keyword

HL-60 cells; apoptosis; (-)-epigallocatechin-3-gallate; caspase-3; poly-(ADP-ribose)-polymerase

MeSH Terms

Apoptosis*
Caspase 3
Catechin
Cell Death
Cell Line
Chromatin
Cytochromes c
Cytoplasm
Cytosol
DNA Fragmentation
Electrophoresis, Agar Gel
HL-60 Cells*
Humans
Hydroxymethylglutaryl-CoA Reductase Inhibitors
Inhibitory Concentration 50
Leukemia, Myeloid
Membranes
Tea
Caspase 3
Catechin
Chromatin
Cytochromes c
Tea
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