Korean J Nutr.
2003 May;36(4):376-381.
Functional Studies of Acyl-CoA Synthetase 4 in the Rat Liver
- Affiliations
-
- 1Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture and Life Science, Biotechnology Research Institute, Chonnam National University, Gwangju, Korea.
Abstract
- Acyl-CoA synthetase 4 (ACS4) is an arachidonate-preferring enzyme abundant in steroidogenic tissues. We examined ACS4 in rat liver, which contains a variety of pathways that use acyl-CoAs, in order to determine subcellular locations. We demonstrate that ACS4 protein was present most abundantly in the mitochondria and to a much lesser extent in the peroxisomes and microsomes. To determined the dietary effects on the level of ACS4 mRNA, northern blotting was carried out using total RNA from the livers of adult male rats fed various diets. Fasting, high fat diet, and fat-free high sucrose diet increased the hepatic level of ACS4 mRNA approximately 2-fold. Furthermore, the levels of ACS4 mRNA were induced by DEHP[Di- (2-ethylhexyl) phthalate]. These data suggest that ACS4 expression in the liver is regulated with a variety of pathways, including beta-oxidation, hormone, and insulin.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Dietary carnosic acid suppresses hepatic steatosis formation via regulation of hepatic fatty acid metabolism in high-fat diet-fed mice
- A Suspect Case of Medium Chanin Acyl-Coenzyme A Dehydrogenase Deficiency with Iron Deficiency Anemia
- Frameshift Mutation of MARS Gene Encoding an Aminoacyl-tRNA Synthetase in Gastric and Colorectal Carcinomas with Microsatellite Instability
- Intractable metabolic acidosis in a child with propionic acidemia undergoing liver transplantation: a case report
- Compound heterozygous mutations of ACADS gene in newborn with short chain acyl-CoA dehydrogenase deficiency: case report and literatures review
