Korean J Otolaryngol-Head Neck Surg.  2002 May;45(5):462-466.

Inhibition of Allergic Response by CpG Motif Immunostimulatory Oligodeoxynucleotide in a Murine Model of Allergic Rhinitis

Affiliations
  • 1Department of Otorhinolaryngology, Inje University, Sanggye Paik Hospital, Seoul, Korea.
  • 2Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University College of Medicine, Seoul, Korea.

Abstract

BACKGROUND AND OBJECTIVES: The classic immunotherapy has the inconvenience and the risk of anaphylaxis. Recent reports revealed that immunostimulatory DNA sequences (CpG motif, ISS-ODN) have been shown to act as strong Th1 response-inducing adjuvants and DNA based vaccination might be an effective therapeutic option for the treatment of allergic disease. In our study, we investigated whether ISS-ODN have anti-allergic effect in the mouse rhinitis model of Dermatophagoides farinae (Der f), the most common allergen in Korea. MATERIALS AND METHOD: C57BL/6 mice were sensitized with crude extract of Der f extract. After injection of ISS-ODN, several parameters of allergic response were evaluated.
RESULTS
Injection with ISS-ODN suppressed itching symptom, eosinophilic infiltration into nasal mucosa and the splenic T cell proliferation after Der f allergen challenge in the murine model of allergic rhinitis. However, injection with ISS-ODN did not show a detectable Der f-specific IgE suppression.
CONCLUSION
Our findings suggested ISS-ODN have an anti-allergic effect in the allergic rhinitis model of Der f allergen. House dust mite allergy can be reduced by the injection with ISS-ODN.

Keyword

Dermatophagoides farinae; Allergic rhinitis; Murine model; CpG motif; Immunostimulatory sequence

MeSH Terms

Anaphylaxis
Animals
Base Sequence
Cell Proliferation
Dermatophagoides farinae
DNA
Eosinophils
Hypersensitivity
Immunoglobulin E
Immunotherapy
Korea
Mice
Nasal Mucosa
Pruritus
Pyroglyphidae
Rhinitis*
Vaccination
DNA
Immunoglobulin E
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