Korean J Physiol Pharmacol.
2000 Dec;4(6):479-486.
The effect of carbon monoxide on contraction, cytosolic Ca2+ level and
ionic currents in guinea pig ileal smooth muscle
- Affiliations
-
- 1Department of Physiology, Yonsei University College of Medicine, 134
Sinchon-dong, Seodaemun-ku, Seoul, South Korea.
tsnam@yumc.yonsei.ac.kr
Abstract
- The aim of this study was to clarify the mechanism of the inhibitory
action of carbon monoxide (CO) on contraction, by measuring cytosolic
Ca2+ level ((Ca2+)i) and ionic currents in guinea-pig ileum. CO (10%)
inhibited 40 mM KCl-induced contraction and this effect was blocked by
ODQ (1 micrometer), a soluble guanylyl cyclase (sGC) inhibitor. CO inhibited
the 40 mM KCl-induced contraction without changing (Ca2+)i. Cumulative
addition of KCl induced a graded increase in (Ca2+)i and muscle
tension. In the presence of CO, cumulative addition of KCl induced
smaller contraction than in the absence of CO. On the other hand, the
increase in (Ca2+)i induced by cumulative addition of KCl was only
slightly decreased in the presence of CO, and the (Ca2+)i-tension
relationship shifted downwards. Using the patch clamp technique with a
holding potential of -60 mV, we found that CO had little effect on the
peak Ba currents (IBa) when voltage was stepped from -60 mV to 0 mV. In
addition, CO showed no effect on the depolarization-activated outward
K+ currents in the all potential ranges. We conclude that CO inhibits
smooth muscle contraction mainly by decreasing the Ca2+ sensitivity of
contractile elements via a cGMP-dependent pathway, not by involving
L-type Ca2+ and outward-potassium currents in guinea-pig ileum.