Immune Netw.  2002 Mar;2(1):12-18. 10.4110/in.2002.2.1.12.

Effect of Interleukin-10 on Lipopolysaccahride/Interferon-gamma- Induced Chemokine Mig Gene Expression

• Choi JH
• Jin JS
• Park HS
• Kim SK
• Lee JY
• Kim HS

• KMID: 1571387
• DOI: http://doi.org/10.4110/in.2002.2.1.12

Abstract

Interferon-gamma (IFN-gamma) is well known as a potent inducer in monokine induced by IFN-gamma (Mig) mRNA expression. Although lipopolysaccharide (LPS) alone is weakly effective on Mig mRNA expression. the stimulation of LPS and IFN-gamma (LPS/IFN-gamma simultaneously has been shown to synergize to produce a high level of Mig mRNA in mouse peritoneal macrophages. In this study, interleukin-10 (IL-10) was found to suppress the LPS/IFN-gamma- induced Mig mRNA expression in cell type- and mouse strain-specific fashion, but IFN-gamma alone-induced Mig mRNA was unaffected by IL-10 under identical experimental conditions. The IL-10-mediated suppression of LPS/IFN-gamma-stimulated Mig mRNA expression was dependent on the concentration of IL-10, and was prevented when the agent was added 2 hours after LPS/IFN-gamma treatment. The suppressive action of IL-10 was dependent on a protein synthesis. However, IL-10 did not reduce the stability of LPS/IFN-gamma-induced Mig mRNA. These data may have important implications for a previously unrecognized role for IL-10 as a regulator of synergistic effect of LPS on the IFN-gamma-induced expression of the Mig gene in macrophages.

Keyword

IL-10; Mig; LPS; IFN-gamma; gene expression

MeSH Terms

Animals
Gene Expression*
Interferon-gamma
Interleukin-10*
Macrophages
Macrophages, Peritoneal
Mice
RNA, Messenger
Interferon-gamma
Interleukin-10
RNA, Messenger