Korean J Physiol Pharmacol.  1997 Oct;1(5):547-553.

Role of tyrosine kinases in vascular contraction in deoxycorticosterone acetate-salt hypertensive rats

• Yeum CH
• Jun JY
• Choi HS

Abstract

It has been known that activation of tyrosine kinases is involved in signal transduction. Role of the tyrosine kinase in vascular smooth muscle contraction was examined in deoxycorticosterone acetate (DOCA)-salt hypertensive rats. Male Sprague-Dawley rats underwent uninephrectomy, one week after which they were subcutaneously implanted with DOCA (200 mg/kg) and supplied with 1% NaCl and 0.2% KCl drinking water for 4-6 weeks. Control rats were treated the same except for that no DOCA was implanted. Helical strips of carotid arteries were mounted in organ baths for measurement of isometric force development. Genistein was used as a tyrosine kinase inhibitor. Concentration-response curves to 5-hydroxytryptamine (5-HT) shifted to the right by genistein in both DOCA-salt hypertensive and control rats. Although the sensitivity to genistein was similar between the two groups, the maximum force generation by 5-HT was less inhibited by genistein in arteries from DOCA-salt hypertensive rats than in those from controls. Genistein-induced relaxations were attenuated in arteries from DOCA-salt rats. Genistein affected the contraction to phorbol 12, 13-dibutyrate (PDBu) neither in DOCA-salt nor in control arteries. These observations suggest that tyrosine kinase is involved in 5-HT-induced vascular contraction, of which role is reduced in DOCA-salt hypertension.

Keyword

Tyrosine kinases; Vascular smooth muscle contraction; Deoxycorticosterone acetate-salt hypertensive rats

MeSH Terms

Animals
Arteries
Baths
Carotid Arteries
Desoxycorticosterone Acetate
Desoxycorticosterone*
Drinking Water
Genistein
Humans
Hypertension
Male
Muscle, Smooth, Vascular
Phosphotransferases*
Protein-Tyrosine Kinases
Rats*
Rats, Sprague-Dawley
Relaxation
Serotonin
Signal Transduction
Tyrosine*
Desoxycorticosterone
Drinking Water
Genistein
Phosphotransferases
Protein-Tyrosine Kinases
Serotonin
Tyrosine