Korean J Gastroenterol.
1999 Oct;34(4):479-488.
Immunohistochemical Reaction of Hepatic Stellate Cells in Dimethylnitrosamine-induced Rat Liver Damage
Abstract
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BACKGROUND/AIMS: Hepatic stellate cell (HSC) is the major source of extracellular matrix in the liver. The purpose of this study was to evaluate the pattern of immunocytochemical expression in HSC during rat hepatofibrosis.
METHODS
Twenty Sprague-Dawley male rats were injected intraperitoneally with dimethylnitrosamine (DMN) three times a week for 3 weeks. The rats were sacrificed weekly after the beginning of experiment. In liver samples, immunohistochemical staining was performed for desmin, alpha-smooth muscle actin (SMA), glial fibrillary acidic protein (GFAP), and neural cell adhesion molecule (N-CAM).
RESULTS
After 1 week from the beginning of experiment, desmin/SMA were evenly expressed throughout hepatic tissue. At 2 weeks, GFAP and N-CAM were present in some HSCs within developing fibrous septa. At 3 weeks showing micronodular cirrhosis, all markers were more accentuated in septa/periseptal areas. At 1 week after stopping the administration of DMN, desmin/SMA/N-CAM were focally expressed within septa and resolved.
CONCLUSIONS
It is suggested that HSCs in rats may display the differential markers according to functional states during experimental hepatofibrosis.
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