J Korean Soc Virol.
1999 Sep;29(3):145-153.
Construction of a Novel Recombinant Baculovirus Producing Polyhedra with Bacillus thuringiensis Cry1Ac Crystal Protein
- Affiliations
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- 1The Research Center for New Bio-Materials in Agriculture, and Division of Applied Biology and Chemistry, College of Agriculture & Life Sciences, Seoul National University, Suwon 441-100, Korea. kskipl@plaza.snu.ac.kr
- 2College of Natural Resources and Life Science, Dong-A University, Pusan 604-714, Korea.
Abstract
- We have now construted a novel recombinant baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV) producing polyhedra with Bacillus thuringiensis (Bt) Cry1Ac crystal protein. The recombinant polyhedra produced by the recombinant baculovirus, Btrus, in insect cells was characterized. The recombinant baculovirus has two independent transcription units in opposite orientations with two promoters, p10 or polyhedrin gene promoter each initiating transcription of either native polyhedrin or fusion protein with polyhedrin and Bt Cry1Ac crystal protein. Suprisingly, this recombinant baculovirus stably produced recombinant polyhedra which were nearly similar to those of wild-type AcNPV. The immunogold staining experiment showed that the recombinant polyhedra were assembled with polyhedrin and Bt Cry1Ac crystal protein, and contained virus paticles. Insecticidal toxicity of recombinant polyhedra of Btrus to the fall webworm, Hyphantrea cunea, was strikingly improved in comparison with the wild-type AcNPV.
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