J Korean Soc Emerg Med.  1999 Sep;10(3):350-362.

Neuroprotective Effects of Intraischemic(1 hour) Moderate Hypothermia in Gerbil Brain Global Ischemic Model

• Kim IB
• Jung KC
• Yim SV
• Jang SJ
• Kim SW

Abstract

BACKGROUND: In animal models of cerebral ischemic-reperfusion has been shown to have a beneficial effect. The object of this study is to compare the effect of pathologic findings between normotheimic and moderate hypothermic group.
METHODS
We investigated the effect of moderate hypothermia induced 1 hour after transient(10 min) both carotid artery occlusion on the extent of ischemic-reperfusion cell damage in Mongolian Gerbil model. The terminal deoxyribonucleotidyl transferase (TdT) -mediated biotin-16-dUTP nick-end labelling(TUNEL staning) are used to detect apoptosis.
RESULTS
1. We suggest that Core body temperature is down to moderate hypothermia(30-32degrees C) beyond 10 minite by selective bain cooling method in Mongolian Gerbil model. 2. By light microscopy, ischemic-reperfusion damage were detected in the hippocampal CA1 pyramidal layer on the 3 day after transient ischemic insult, which showed chrosomal condensation and cytoplasmic eosinophilia. Ischemic-reperfusion cells were increased in the CA1 region on the 5 day. Apoptotic cells of the CA1 neurons seen by TUNEL staining than ischemic neurons seen by Hematoxylin-eosin staining were investigate 3 and 5 days after ischemic-reperfusion insult.
CONCLUSION
We suggest that is not neuroprotective effects of Intraischemic(1 hour) moderate hypothermia in Gerbil brain global ischemic-reperfusion model.

MeSH Terms

Apoptosis
Body Temperature
Brain*
Carotid Arteries
Cytoplasm
DNA Nucleotidylexotransferase
Eosinophilia
Gerbillinae*
Hypothermia*
In Situ Nick-End Labeling
Microscopy
Models, Animal
Neurons
Neuroprotective Agents*
DNA Nucleotidylexotransferase
Neuroprotective Agents