Korean J Med Mycol.  2004 Mar;9(1):12-27.

The Mycological and Molecular Biological Studies on Arthroderma benhamiae Isolated for the First Time in Korea

Affiliations
  • 1Department of Dermatology, Daegu Catholic University School of Medicine, Korea. admin@tcmcch.co.kr
  • 2Department of Dermatology, Kyungpook National University School of Medicine, Korea.
  • 3Department of Dermatology, College of Medicine, Yeungnam University, Korea.
  • 4Catholic Skin Clinic, Daegu, Korea.

Abstract

BACKGROUND
Arthroderma (A.) benhamiae, one of three telemorphs of Trichophyton (T.) mentagrophytes, has not been isolated until recently in Korea, but in Japan the first report on it was made in 1998. OBJECTIVE: To identify A. benhamiae for the first time in Korea. METHODS: Twelve strains suggestive of A. benhamiae grossly and microscopically were detected among 1, 059 of T. mentagrophytes isolated at the Catholic Skin Clinic, Daegu, from 1998 to 2000. They were examined by mating tests and molecular methods. In mating tests, they were respectively crossed with "+" and "-" tester strains of A. vanbreuseghemii, A. benhamiae African race, A. benhamiae Americano-European race. Molecular methods included the sequence analysis of internal transcribed spacer 2 (ITS2) region of ribosomal DNA and chitin synthase 1 (CHS1) gene, and random amplified DNA polymorphism (RAPD) with random primer OPAO-15 (5'-GAA GGC TCC C-3'). RESULTS: Mating tests revealed that 12 strains of T. mentagrophytes consisted of 6 of A. benhamiae, 4 of A. vanbreuseghemii and 2 of indeterminate. Six strains of A. benhamiae, all isolated from the patients with a history of contact with rabbits, included 1 of Americano-European race and 5 of African race of Americano-European race and 5 of African race. One clinical isolate, a strain of A. benhamiae Americano-European race "-" in mating tests, was almost identical with the standard strains of A. benhamiae Americano-European race with molecular methods as 99.2% (351/354) similarity of ITS2 sequence, 99.1% (313/ 316) similarity of CHS1 sequence, and similar RAPD pattern. Five clinical strains of A. benhamiae African race were identical with standard strains in ITS2 and CHS1 gene and RAPD pattern. CONCLUSION: The first isolation of 6 strains of A. benhamiae in Korea was reported. Five were identified as A. benhamiae African race "+", and 1 as A. benhamiae Americano-European race "-".

Keyword

Trichophyton mentagrophytes; Arthroderma benhamiae; Clinical isolates; Korea

MeSH Terms

Arthrodermataceae*
Chitin Synthase
Continental Population Groups
Daegu
DNA
DNA, Ribosomal
Humans
Japan
Korea*
Rabbits
Sequence Analysis
Skin
Trichophyton
Chitin Synthase
DNA
DNA, Ribosomal
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