Korean J Ophthalmol.  2013 Feb;27(1):48-51. 10.3341/kjo.2013.27.1.48.

Effect of Heat Shock Protein 72 Expression on Etoposide-induced Cell Death of Rat Retinal Ganglion Cells

• Sohn S
• Im JE
• Kim TE
• Kee C

• KMID: 1501796
• DOI: http://doi.org/10.3341/kjo.2013.27.1.48

Abstract

PURPOSE
To assess whether the expression of heat shock protein 72 (Hsp72) protects rat retinal ganglion cells (RGC-5) from apoptotic cell death.
METHODS
Hsp72 expression in RGC-5 cells transduced with replication-deficient recombinant adenovirus was analyzed by Western blot analysis and immunofluorescence. The effect of Hsp72 expression on etoposide-induced apoptotic cell death was examined by microscopic analysis and confirmed by cell proliferation assay.
RESULTS
Western blot analysis and immunofluorescence clearly showed adenovirus-mediated Hsp72 expression in RGC-5 cells. Treatment with etoposide resulted in the death of a proportion of the cells by apoptosis. However, this apoptotic cell death was significantly reduced in cells expressing Hsp72, with the reduction in cell death correlating to the level of Hsp72 expression.
CONCLUSIONS
Over-expression of Hsp72 alone is sufficient to rescue neuronal cells from apoptotic cell death, suggesting that fine-tuning its expression may be an effective neuroprotective approach in retinal degenerative disease.

Keyword

Adenoviral vector; Apoptosis; Glaucoma; Hsp72 heat-shock proteins; Retinal ganglion cells

MeSH Terms

Animals
Blotting, Western
Cell Death/*genetics
Cell Survival
Cells, Cultured
DNA/*genetics
Disease Models, Animal
Etoposide/toxicity
*Gene Expression Regulation
HSP72 Heat-Shock Proteins/biosynthesis/*genetics
Immunohistochemistry
Rats
Retinal Degeneration/*genetics/metabolism/pathology
Retinal Ganglion Cells/drug effects/*metabolism/pathology
HSP72 Heat-Shock Proteins
Etoposide
DNA