Exp Neurobiol.  2011 Dec;20(4):169-175. 10.5607/en.2011.20.4.169.

Chlorogenic Acid Improves Neuroprotective Effect of PEP-1-Ribosomal Protein S3 Against Ischemic Insult

Affiliations
  • 1Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 200-702, Korea. sychoi@hallym.ac.kr
  • 2Department of Anatomy, College of Medicine, Soonchunhyang University, Cheonan 330-090, Korea.
  • 3Department of Food Science and Nutrition & RIC Center, Hallym University, Chunchon 200-702, Korea.
  • 4School of Life Science and Biotechnology, Korea University, Seoul 136-701, Korea.

Abstract

Chlorogenic acid (CGA) possesses various biological activities such as anti-oxidant, anti-inflammatory, and anti-diabetic activities. In the present study, we examined the effect of CGA on the transduction efficiency of PEP-1-ribosomal protein S3 (PEP-1-rpS3) into cells and brain tissues, and its neuroprotective potential against ischemia/reperfusion. We found that, in the presence of CGA, the transduction efficiency of PEP-1-rpS3 into astrocytes and the CA1 region of the hippocampus was enhanced, compared to its transduction in the absence of CGA. Also, cell viability data demonstrated that the sample treated with CGA + PEP-1-rpS3 exhibited improved cell viability against hydrogen peroxide (H2O2)-induced toxicity more significantly than the sample treated with PEP-1-rpS3 alone. Also, in a gerbil ischemia model, data demonstrated that following the ischemic insult, the group treated with PEP-1-rpS3 + CGA showed markedly enhanced protection of neuron cells in CA1 region of hippocampus, compared to those treated with CGA or PEP-1-rpS3 alone. Taken together, these results suggest that CGA may improve the transduction efficiency of protein transduction domain (PTD) fusion proteins into target cells or tissues, thereby enhancing their therapeutic potential against various diseases.

Keyword

chlorogenic acid; PEP-1-ribosomal protein S3; protein transduction; ischemic insult

MeSH Terms

Astrocytes
Brain
Cell Survival
Chlorogenic Acid
Gerbillinae
Hippocampus
Hydrogen Peroxide
Ischemia
Neurons
Neuroprotective Agents
Proteins
Chlorogenic Acid
Hydrogen Peroxide
Neuroprotective Agents
Proteins
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