Ann Clin Microbiol.  2013 Sep;16(3):126-133. 10.5145/ACM.2013.16.3.126.

Molecular Characteristics of blaOXA-23-Producing Acinetobacter baumannii Isolated from a University Hospital

• Jang IH
• Park SD
• Uh Y
• Lee GS
• Kim JB
• Choi I

• KMID: 1494329
• DOI: http://doi.org/10.5145/ACM.2013.16.3.126

Abstract

BACKGROUND
Multi-drug resistant (MDR) Acinetobacter baumannii has emerged as a significant infectious agent in hospitals worldwide. The purpose of this study was to determine the molecular characterization of MDR A. baumannii clinical isolates.
METHODS
Two hundred eighty-five strains of non-duplicated A. baumannii collected from March to November 2011 from a university hospital laboratory located in the Wonju area of the Gangwon province of Korea were analyzed for MDR genes.
RESULTS
All of the 285 imipenem-resistant A. baumannii isolates were encoded by a blaOXA-23-like gene, and all isolates with the blaOXA-23-like gene had the upstream element ISAba1. The 16S rRNA methylase gene armA was detected in 153 (50.2%) clinical isolates, but rmtA, rmtB, rmtC, rmtD and npmA were not detected in any isolates in the present study. The gene encoding aac(6')-Ib was the most prevalent aminoglycoside-modifying enzyme. The sequencing data for the quinolone resistance-determining region of gyrA and parC revealed the presence of Ser (TCA) 83 to Leu (TTA) and Ser (TCG) 80 to Leu (TTG) substitutions. All but one of the 285 A. baumannii isolates showed similar band patterns on repetitive extragenic palindromic-PCR profiles.
CONCLUSION
The molecular characteristics of the resistance genes of MDR A. baumannii isolates obtained from the Wonju area of Gangwon province were similar to those of other areas in Korea.

Keyword

Acinetobacter baumannii; Beta-lactamases; Genes, Multidrug resistance; Imipenem

MeSH Terms

Acinetobacter
Acinetobacter baumannii
beta-Lactamases
Genes, MDR
Imipenem
Korea
Laboratories, Hospital
Methyltransferases
Imipenem
Methyltransferases
beta-Lactamases

Figure

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  Fig. 1. Dendrogram of the similarity index by unweighted pair group method with mathematical averaging (UPGMA) among two hundred sixty-five A. baumannii isolates provided by REP-PCR analysis.

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