J Vet Sci.  2013 Mar;14(1):1-6. 10.4142/jvs.2013.14.1.1.

C6, a new monoclonal antibody, reacts with the follicle-associated epithelium of calf ileal Peyer's patches

Affiliations
  • 1Department of Veterinary Anatomy, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan. yasudaja@cc.miyazaki-u.ac.jp
  • 2Department of Veterinary Anatomy and Cell Biology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
  • 3AgResearch, The Hopkirk Research Institute, Grasslands Research Centre, Palmerston North 4442, New Zealand.

Abstract

The follicle-associated epithelium (FAE) of Peyer's patches (PPs) contains M cells that are important for reducing mucosal immune responses by transporting antigens into the underlying lymphoid tissue. We generated a monoclonal antibody (C6) that reacted with the FAE of calf ileal PPs, and analyzed the characteristics of C6 using immunohistochemistry and Western blotting. FAE of the ileal PP was stained with C6 during both late fetal developmental and postnatal stages. Neither the villous epithelial cell nor intestinal crypt basal cells were stained at any developmental stage. During the prenatal stages, FAE of the jejunal PP was C6-negative. However, a few C6-positive cells were distributed diffusely in some FAE of the jejunal PPs during the postnatal stages. The protein molecular weight of the antigen recognized by C6 was approximately 45 kDa. These data show that C6 is useful for identifying the FAE in ileal PPs and further suggest that differentiation of the FAE in these areas is independent of external antigens.

Keyword

bovine; follicle-associated epithelium; monoclonal antibody; Peyer's patch
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