Pediatr Allergy Respir Dis.  2009 Sep;19(3):233-240.

Effects of Cooking Methods on Peanut Allergenicity

Affiliations
  • 1Department of Pediatrics, Bundang Jesaeng General Hospital, Sungnam, Korea.
  • 2Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. snuhan@unitel.co.kr

Abstract

PURPOSE
Peanut allergy is a major cause of fatal food-induced anaphylaxis. Cooking methods can affect the allergic properties of peanut proteins. The aim of this study was to determine the allergenicity of peanut according to cooking methods.
METHODS
Eight kinds of peanut were included in the study: raw peanut, boiled peanut, roasted peanut (10 min, 20 min and 30 min), peanut butter, fried peanut and vinegarish peanut. The proteins were extracted with PBS and analyzed using the SDS-PAGE IgE immunoblot assay with pooled sera from 8 patients with atopic dermatitis. These patients had peanut- specific IgE levels greater than 15 kU/L, which were measured by the CAP-FEIA.
RESULTS
The SDS-PAGE IgE immunoblot assay revealed more intense protein bands of Ara h 2 in roasted peanut and peanut butter than in raw, boiled, fried and vinegarish peanut. The protein band of Ara h 1 was not undetected in fried and vinegarish peanut. Ara h 3 had a stable band pattern in all samples, but there was the most prominent band at 37-40 kDa in vinegarish peanut. The IgE immunoblot assay revealed that 10 min roasted peanut had more IgE binding to Ara h 2, and there was no IgE binding to Ara h 1 in fried and vinegarish peanut. In vinegarish peanut, there was almost no IgE binding to it.
CONCLUSION
The results of this study suggest that the roasted peanut may increase the allergenicity of Ara h 2 as compared to Ara h 1. Fried and vinegarish peanut may reduce the allergenicity of peanut.

Keyword

Peanut allergy; Ara h 1 allergen; peanut; Ara h 2 allergen; peanut; Ara h 3 allergen; peanut

MeSH Terms

Anaphylaxis
Butter
Cooking
Dermatitis, Atopic
Electrophoresis, Polyacrylamide Gel
Humans
Immunoglobulin E
Peanut Hypersensitivity
Proteins
Butter
Immunoglobulin E
Proteins
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