Korean J Anat.  2009 Sep;42(3):179-185.

Effect of Water Extract of Papaya on RANKL-induced Osteoclast Differentiation

Affiliations
  • 1Department of Anatomy, School of Medicine, Wonkwang University, Iksan, Jeonbuk 570-749, Korea. JJkim@wku.ac.kr
  • 2Department of Rheumatology, School of Medicine, Wonkwang University, Iksan, Jeonbuk 570-749, Korea.
  • 3Department of Oriental Pharmacy, School of Pharmacy, Wonkwang University, Iksan, Jeonbuk 570-749, Korea.

Abstract

Balance between bone-resorbing osteoclats and bone-forming osteoblasts is important in bone homeostasis. In particular, increased osteoclast formation and activity are responsible for bone diseases such as osteoporosis, rheumatoid arthritis, periodontal disease. Natural metabolites of plants have recently received much attention as lead compounds for the development of novel therapeutic strategy. The purpose of this study was to search the natural products to inhibition osteoclast differentiation. Water extract of papaya significantly inhibited receptor activator of nuclear factor-kappaB ligand (RANKL)-induced osteoclast differentiation in bone marrow macrophages (BMMs) in a dose dependent manner. However, water extract of papaya did not affect cytotoxicity when compared with control. Water extract of papaya inhibited the phosphorylation of p38 and JNK induced by RANKL. The mRNA expression of c-Fos, NFATcl, TRAP and OSCAR induced by RANKL was inhibited by water extract of papaya treatment. Also, water extract of papaya suppressed the protein expression of c-Fos and NFATc1 in BMMs treated with RANKL. Taken together, these results suggest that papaya may be a useful drug in the treatment of bone-related disease.

Keyword

Bone; Papaya; Osteoclast; RANKL

MeSH Terms

Arthritis, Rheumatoid
Biological Agents
Bone Diseases
Bone Marrow
Carica
Homeostasis
Macrophages
Osteoblasts
Osteoclasts
Osteoporosis
Periodontal Diseases
Phosphorylation
RANK Ligand
RNA, Messenger
Water
Biological Agents
RANK Ligand
RNA, Messenger
Water
Full Text Links
  • KJA
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2022 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr