Lab Anim Res.  2010 Mar;26(1):55-62. 10.5625/lar.2010.26.1.55.

Effect of Sodium Silicate on Hair Growth in C57BL/6 Mice

• Hue JJ
• Jo BK
• Kang BS
• Kim JH
• Nam SY
• Yun YW
• Kim JS
• Jeong JH
• Lee SH
• Ahn JB
• Lee BJ

• KMID: 1458940
• DOI: http://doi.org/10.5625/lar.2010.26.1.55

Abstract

A promoting effect of Na2SiO3 on hair regrowth was investigated using an animal model of C57BL/6 mice. There were four experimental groups including distilled water (DW, a negative control), 5% minoxidil (MXD, a positive control), 50% Na2SiO3, and 100% Na2SiO3 solution. The animals were shaved with an electric clipper and then test solutions applied daily with a volume of 0.2 ml per to the dorsal skin of mice for 3 weeks. Body weight and food and water consumption were measured weekly. Photographs of hair regrowth were taken at experimental day 0, 4, 7, 10, 14, 17, and 21. Activities of alkaline phosphatase and gamma-glutamyl transpeptidase as well as expressions of growth factors were also determined in the dorsal skin of mice. The animal body weight were not significantly changed among the experimental groups. The MXD and Na2SiO3 accelerated hair regrowth compared with DW. The elongation of hair follicles were evidently observed in MXD and 50 or 100% Na2SiO3 groups. MXD significantly increased gamma-glutamyl transpeptidase at day 14, compared with DW (P<0.05). But the activities of alkaline phosphatase and gamma-glutamyl transpeptidase were not significantly increased in Na2SiO3 groups, compared with DW. The expression of epidermal growth factor was significantly increased in MXD and Na2SiO3 groups, compared with DW (P<0.05). The expression of vascular endothelial growth factor was not significantly changed by MXD or Na2SiO3 treatments. The expression of transforming growth factor (TGF)-beta1 was clearly decreased in MXD and Na2SiO3 groups, compared with DW. These results indicate that Na2SiO3 may have a hair growth-promoting activity and it can be used for treatment of alopecia or boldness in humans.

Keyword

Na2SiO3; hair growth; ALP; gamma-GT; EGF; VEGF; minoxidil

MeSH Terms

Alkaline Phosphatase
Alopecia
Animals
Body Weight
Drinking
Epidermal Growth Factor
gamma-Glutamyltransferase
Hair
Hair Follicle
Humans
Intercellular Signaling Peptides and Proteins
Mice
Minoxidil
Models, Animal
Silicates
Skin
Sodium
Transforming Growth Factors
Vascular Endothelial Growth Factor A
Water
Alkaline Phosphatase
Epidermal Growth Factor
Intercellular Signaling Peptides and Proteins
Minoxidil
Silicates
Sodium
Transforming Growth Factors
Vascular Endothelial Growth Factor A
Water
gamma-Glutamyltransferase

Figure

• 

  Figure 1. Change in body weight of C57BL/6 mice for 3 weeks. The animals were shaved with electric clipper and the test compounds applied with 0.2 mL per to the dorsal skin of mice for 3 weeks. D.W: distilled water, MXD: 5% minoxidil. Date represent mean±SD (n=5).

• 

  Figure 2. Photograph of hair regrowth in C57BL/6 mice. The animals were shaved with electric clipper and the test compounds applied with 0.2 mL per to the dorsal skin of mice for 3 weeks. The photographs were taken day 0, 4, 7, 10, 14, 17, and 21. DW: distilled water, MXD: 5% minoxidil.

• 

  Figure 3. Histological change of hair follicles in the dorsal skin of C57BL/6 mice (H&E, ×40). The animals were shaved with electric clipper and the test compounds applied with 0.2 mL per to the dorsal skin of mice for 3 weeks. D.W: distilled water, MXD: 5% minoxidil.

• 

  Figure 4. Alkaline phosphatase (ALP) changes in the dorsal tissue of C57BL/6mice. The animals were shaved with electric clipper and the test compounds applied with 0.2 mL per to the dorsal skin of mice for 3 weeks. D.W.: Distilled water, MXD: Minoxidil. Data represent mean±SD (n=5).

• 

  Figure 5. γ-glutamyl transpeptidase (γ-GT) changes in the dorsal tissue of C57BL/6 mice. The animals were shaved with electric clipper and the test compounds applied with 0.2 mL per to the dorsal skin of mice for 3 weeks. DW: Distilled water, MXD: Minoxidil. Data represent mean±SD (n=5). ∗Significantly different from the D.W. group (P<0.05).

• 

  Figure 6. Activities of Endothelial growth factor (EGF) in the dorsal tissue of C57BL/6 mice. The animals were shaved with electric clipper and the test compounds applied with 0.2 mL per to the dorsal skin of mice for 3 weeks. D.W.: Distilled water, MXD: 5% Minoxidil. Data represent mean±SD (n=5). ∗Significantly different from the D.W. group (P<0.05).

• 

  Figure 7. Activities of vascular endothelial growth factor (VEGF) in the dorsal tissue of C57BL/6 mice. The animals were shaved with electric clipper and the test compounds applied with 0.2 mL per to the dorsal skin of mice for 3 weeks. D.W.: Distilled water, MXD: 5% Minoxidil. Data represent mean±SD (n=5).

• 

  Figure 8. Expression of TGF-β in the dorsal skin of C57BL/6 mice. The animals were shaved with electric clipper and the test compounds applied with 0.2 mL per to the dorsal skin of mice for 3 weeks. D.W.: Distilled water, MXD: 5% Minoxidil.

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