Lab Anim Res.  2010 Mar;26(1):1-6. 10.5625/lar.2010.26.1.1.

Anti-hepatofibrogenic Effect of Turnip Water Extract on Thioacetamide-induced Liver Fibrosis

• Li L
• Park DH
• Li YC
• Park SK
• Lee YL
• Choi HM
• Han DS
• Yang HJ
• Lee EH
• Jang HK
• Kim YJ
• Jang JJ
• Lee MJ

• KMID: 1458933
• DOI: http://doi.org/10.5625/lar.2010.26.1.1

Abstract

Liver fibrosis is a chronic liver disease and lots of people in Korea are suffered. There are many efforts to find candidates to suppress liver fibrogenesis and several chemical-induced model or bile duct ligation model have been used to research and develop hepatic fibrogenic suppressor. From the previous study about functional effects of turnip which cultivated in Kangha Island, we got the feasibility which turnip might be able to inhibit heptatic fibrogenesis. TAA is a representative hepatic fibrosis inducer, repeated 7-weeks i.p. injection of it results in hepatic fibrosis. We compared the level of hepatic fibrosis in TAA-turnip group, TAA group, and vehicle control group. Nodules-formed by TAA were observed; they were rarely shown in vehicle control group, observed in most area in TAA group, but only shown in periportal regions in TAA-turnip group. These results were confirmed through Masson's trichrom stain; fibrous structures increased in TAA group (fibrosis score: 4) but significantly decreased in TAA-turnip group (fibrosis score: 2-3). In conclusion, we got the result that turnip water extract has a potency to protect TAA-induced hepatic fibrogenesis but it is necessary further study to find its mechanism.

Keyword

Turnip water extract; thioacetamide (TAA); hepatofibrogenic animal model; nodular formation

MeSH Terms

Bile Ducts
Brassica napus
Fibrosis
Korea
Ligation
Liver
Liver Cirrhosis
Liver Diseases
Water
Water

Figure

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  Figure 1. The protocol of TAA-induced hepatic fibrogenetic animal model. Arrows mean administrated a intraperitoneal injection of TAA at 200 mg/kg a week.

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  Figure 2. TAA treated groups showed lower body weight gain compared to TAA non-treated group during the experiment. All values are expressed as mean±SD. ∗P<0.05, TAA untreated groups vs. TAA treated groups.

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  Figure 3. Physiological changes by TAA treatment or untreatment (A) Relative liver weight of experimental animals. TAA treated groups is larger than that in TAA untreated groups. All values are expressed as mean±SD. P<0.05, TAA untreated groups vs. TAA treated groups. (B) Serological analysis of liver enzymes in TAA treated rat liver with turnip water extract. It is not different from activities of serum enzymes of control and those of turnip water extract treatment group. All values are expressed as mean±SD. P<0.05, TAA untreated groups vs. TAA treated groups.

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  Figure 4. Anti-hepatofibrogenic effects of turnip water extract (A) Photos of livers in control and turnip water extract treatment group are normal but that in TAA treatment is shown typical hepatic fibrosis. Turnip water extract almost inhibits TAA-induced nodular formation and fibrosis. (B) Fibrosis scores in each group. Semi-quantitative scoring system is adopted to evaluate the degree of liver fibrosis (0: no, score 1: portal fibrosis, score 2: periportal fibrosis, score 3: septal fibrosis, score 4: cirrhosis). Mean of fibrosis scores in TAA treatment group is 4 but turnip water extract decreases mean of fibrosis scores by 2-3. All values are expressed as mean±SD. P<0.05, TAA untreated groups vs. TAA treated groups.

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