Tuberc Respir Dis.  2010 Jun;68(6):334-344. 10.4046/trd.2010.68.6.334.

Moxifloxacin Ameliorates Oleic Acid-induced Acute Lung Injury by Modulation of Neutrophilic Oxidative Stress in Rats

Affiliations
  • 1Department of Physiology, Daegu Catholic University School of Medicine, Daegu, Korea. leeym.cu.ac.kr

Abstract

BACKGROUND
Based on the known immunoregulatory functions of moxifloxacin on phagocytes, the therapeutic effect of moxifloxacin on oleic acid (OA)-induced acute lung injury (ALI) was investigated.
METHODS
Moxifloxacin (10 mg/kg) was given to male Sprague-Dawley rats that had been given oleic acid (OA, 30 microliter) intravenously. Five hours after OA injection, parameters demonstrating ALI were assessed to measure the effects of moxifloxacin on acute lung injury.
RESULTS
The pathological findings of OA-induced ALI's was diminished by moxifloxacin. Through ultrastructural and CeCl3 EM histochemistry, moxifloxacin was confirmed to be effective in decreasing oxidative stress in the lung as well. Indices of ALI, such as lung weight/body weight ratio, protein content in bronchoalveolar lavage fluid, and lung myeloperoxidase were decreased by moxifloxacin. In diaminobenzidine immunohistochemistry, fluorescent immunohistochemistry, and Western blotting of the lung, moxifloxacin had decreased the enhanced expression of secretory phospholipase A2 (sPLA2) by OA.
CONCLUSION
We concluded that moxifloxacin was effective in lessening acute inflammatory pulmonary edema caused by OA, by inhibiting the neutrophilic respiratory burst, which was initiated by the activation of sPLA2.

Keyword

Acute Lung Injury; Neutrophils; Oleic Acid; Free Radicals; Phospholipases A2, Secretory

MeSH Terms

Acute Lung Injury
Animals
Aza Compounds
Blotting, Western
Bronchoalveolar Lavage Fluid
Cerium
Free Radicals
Humans
Immunohistochemistry
Lung
Male
Neutrophils
Oleic Acid
Oxidative Stress
Peroxidase
Phagocytes
Phospholipases A2, Secretory
Pulmonary Edema
Quinolines
Rats
Rats, Sprague-Dawley
Respiratory Burst
Aza Compounds
Cerium
Free Radicals
Oleic Acid
Peroxidase
Phospholipases A2, Secretory
Quinolines
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