Int J Oral Biol.  2010 Dec;35(4):191-195.

Antimicrobial Effects of Oleanolic Acid against Streptococcus mutans and Streptococcus sobrinus Isolated from a Korean Population

• Kim MJ
• Kim CS
• Ha WH
• Kim BH
• Lim YK
• Park SN
• Cho YJ
• Kim M
• Ko JH
• Kwon SS
• Ko YM
• Kook JK

Abstract

Oleanolic acid is a natural triterpenoid that exists widely in foods and some medicinal herbs. The purpose of this study was to determine the antimicrobial activity of oleanolic acid against Streptococcus mutans strains isolated from a Korean population. Antimicrobial activity against these bacteria was evaluated by minimal inhibitory concentration (MIC) and time kill curves. The tolerance of human gingival fibroblasts and human periodontal ligaments to oleanolic acid was tested using a methyl thiazolyl tetrazolium (MTT) assay. The MIC90 value of oleanolic acid for both S. mutans and S. sobrinus isolated from Koreans was 8 microg/ml. Oleanolic acid showed bactericidal effects against S. mutans ATCC 25175T and S. sobrinus ATCC 33478T at 1 x MIC (8 microg/ml) and had no cytotoxic effects against KB cells at this dose. The results suggest that oleanolic acid could be useful in the future development of oral hygiene products for the prevention of dental caries.

Keyword

Antimicrobial effect; oleanolic acid; Streptococcus mutans

MeSH Terms

Bacteria
Dental Caries
Fibroblasts
Humans
KB Cells
Oleanolic Acid
Oral Hygiene
Periodontal Ligament
Plants, Medicinal
Streptococcus
Streptococcus mutans
Streptococcus sobrinus
Oleanolic Acid