Korean Circ J.  2010 Sep;40(9):454-458. 10.4070/kcj.2010.40.9.454.

Exercise Training Improves Age-Related Myocardial Metabolic Derangement: Proton Magnetic Resonance Spectroscopy Study in the Rat Model

• Choi SI
• Chang HJ
• Chun EJ
• Cho SB
• Kim ST
• Yoon YE
• Chang SA
• Kim JH
• Kim CH
• Lim TH

• KMID: 1456138
• DOI: http://doi.org/10.4070/kcj.2010.40.9.454

Abstract

BACKGROUND AND OBJECTIVES
The objective of this study was to determine whether long-term exercise training will improve age-related cardiac metabolic derangement using proton magnetic resonance (MR) spectroscopy.
MATERIALS AND METHODS
Young and old male Fischer 344 rats were assigned to sedentary controls groups {young control (YC) group-3 months of age: YC, n=10; old control (OC) group-22 months of age: OC, n=10}, and an exercise training group (OT, n=5). After 12-week of treadmill exercise training, MR spectroscopy at 4.7 T was performed to assess myocardial energy metabolism: measurements of myocardial creatine-to-water ratio (Scr/Sw) were performed using the XWIN-NMR software.
RESULTS
Exercise capacity was 14.7 minutes greater in OT than that in OC (20.1+/-1.9 minutes in OT, 5.4+/-2.3 minutes in OC; p<0.001). The 12-week exercise training rendered the old rats a maximum exercise capacity matching that of untrained YC rats (17.9+/-1.5 minutes in YC, 20.1+/-1.9 minutes in OT; p>0.05). The creatine-to-water ratios in the interventricular septa of YC did not differ significantly from that of OT (0.00131+/-0.00025 vs. 0.00127+/-0.00031; p=0.37). However, OC showed significant reduction in creatine-to-water ratio compared to OT (0.00096+/-0.00025 vs. 0.00127+/-0.00031; p<0.001). Mean total creatine concentrations in the myocardium were similar between YC and OT (13.3 +/-3.6 vs. 11.5+/-4.1 mmol/kg wet weight; p=0.29). In contrast, the mean total creatine concentration of OC was significantly reduced compared to OT (6.8+/-3.2 vs. 11.5+/-4.1 mmol/kg wet weight; p=0.03).
CONCLUSION
Our findings suggest that long-term exercise training in old rats induced prevention of age-related deterioration in myocardial metabolism.

Keyword

Magnetic resonance spectroscopy

MeSH Terms

Animals
Creatine
Humans
Magnetic Resonance Spectroscopy
Magnetics
Magnets
Male
Myocardium
Protons
Rats
Spectrum Analysis
Creatine
Protons

Figure

• 

  Fig. 1 1H-spectra using a 2×2×3 mm voxel (white box) positioned in the septum (A) were obtained from a heart of the young control group (B, left), the old control group (B, middle), and the exercise training group (B, right). The spectrum of young control group and the exercise training group show no significant difference in myocardial creatine-to-water ratio. However, decreased creatine-to-water ratio was detectable in the old control group.

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