J Bacteriol Virol.  2011 Sep;41(3):183-187. 10.4167/jbv.2011.41.3.183.

Protein Expression of the Human Norovirus Capsid Gene using the Baculovirus Expression System

• Jin JY
• Park CJ
• Park SW
• Paik SY

• KMID: 1449893
• DOI: http://doi.org/10.4167/jbv.2011.41.3.183

Abstract

Human norovirus (HuNoV) is the major etiological agent of nonbacterial gastroenteritis worldwide. However, due to the absence of a rapid and sensitive diagnostic system, detection and monitoring have been limited. The HuNoV genome is composed of three open reading frames (ORFs). And major capsid protein, ORF2, is designated as a viral protein 1 (VP1). In this study, the baculovirus expression system was used for expression of the HuNoV capsid protein, VP1. Recombinant baculoviruses can be used as potent tools in HuNoV studies.

Keyword

Human norovirus; Baculovirus expression system

MeSH Terms

Baculoviridae
Capsid
Capsid Proteins
Gastroenteritis
Genome
Humans
Norovirus
Open Reading Frames
Capsid Proteins

Figure

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  Figure 1. Amplified Fragments of ORF2 S-P1 (543 bp), ORF2 epi (1,224 bp), and ORF2 P2 (347 bp) of the HuNoV capsid gene by RT-PCR (M: DNA size Marker).

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  Figure 2. The Gateway cloning strategy. The process comprises of two recombination processes; TOPO cloning reaction (A) and LR recombinant reaction (B).

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  Figure 3. Analysis of expression of recombinant protein from insect cells by SDS-PAGE; ORF2 epi (54 kDa), ORF2 S-P1 (29 kDa), and ORF2 P2 (23.4 kDa).

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