Korean J Physiol Pharmacol.  2011 Aug;15(4):241-244. 10.4196/kjpp.2011.15.4.241.

Molecular Characterization and Mitogenic Activity of a Lectin from Purse Crab Philyra Pisum

Affiliations
  • 1Physical Pharmacy Laboratory, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea. hahyung@cau.ac.kr

Abstract

A lectin from the hemolymph of purse crab, Philyra pisum, was found to have anti-proliferative activity on human lung cancer cells by our laboratory. In this study, P. pisum lectin (PPL) was molecularly characterized including molecular mass, amino acid sequences, amino acid composition, and the effects of metal ions, temperature, and pH on the activity. We found that PPL showed mitogenic activity on human lymphocytes and BALB/c mouse splenocytes. The mitogenic activity (maximum stimulation index, SI=9.57+/-0.59) of PPL on human lymphocytes was higher than that of a standard well-known plant mitogen, concanavalin A (maximum SI=8.80+/-0.59). The mitogenic activity mediated by PPL is required for optimum dosing, and higher or lower concentrations caused decreases in mitogenic response. PPL also induced mitogenic activity on mouse splenocytes, however, the maximum SI (1.77+/-0.09) on mouse splenocytes of PPL was lower than that (2.14+/-0.15) of concanavalin A. In conclusion, PPL is a metal ion-dependent monomer lectin with mitogenic activity, and could be used as a lymphocyte or splenocyte stimulator.

Keyword

Crab; Lectin; Molecular characterization; Mitogenic activity; Lymphocyte; Splenocyte

MeSH Terms

Amino Acid Sequence
Animals
Concanavalin A
Hemolymph
Humans
Hydrogen-Ion Concentration
Ions
Lung Neoplasms
Lymphocytes
Mice
Plants
Concanavalin A
Ions

Figure

  • Fig. 1. Electrophoretic analyses of PPL. (A) Lane 1, molecular standard marker; lane 2, PPL; lane 3, PPL with 2-mercaptoethanol; (B) lane 4, PPL; lane 5, pI standard markers.


Reference

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