Korean J Blood Transfus.  2011 Aug;22(2):99-109.

Comparison of Methods for Detecting Bacterial Contamination in Platelet Concentrates

Affiliations
  • 1Blood Transfusion Research Institute, Korean Red Cross, Seoul, Korea. sykwon@redcross.or.kr
  • 2Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.

Abstract

BACKGROUND
Bacterial contamination of platelets represents the highest infectious risk for a transfusion. In this study, we evaluated 2 culture-based systems that have been approved by the US FDA for bacterial screening.
METHODS
Platelet concentrates were inoculated with 5 bacterial species to give a final concentration of 10(0), 10(1) and 10(2) CFU/mL. Samples for culture were taken immediately after inoculation (0 hr sample) and after 24 hrs (24 hr sample). For the BacT/ALERT 3D system, a 10 mL sample was inoculated into an aerobic culture bottle and incubated for 7 days. For the Pall eBDS system, 3 mL samples were taken from the 0 hr and 24 hr samples, respectively. The samples were incubated for 24 hrs and 30 hrs.
RESULTS
Both systems detected all inoculated units both in the 0 hr and 24 hr samples, except for units inoculated with K. pneumoniae. Eleven units out of 30 units inoculated with K. pneumoniae were detected by the BacT/ALERT 3D system in the 24 hr samples. The Pall eBDS system detected 8 of 30 units in the 24 hr samples.
CONCLUSION
Implementation of either system will decrease the risk of transfusing bacterially contaminated platelets. However, testing for bacterial contamination will not completely prevent septic transfusion reactions; pathogen inactivation that is now available should also be considered as an alternative method to reduce the risk of bacterial contamination.

Keyword

Bacterial contamination; Platelets; BacT/ALERT 3D system; Pall eBDS system

MeSH Terms

Benzeneacetamides
Blood Platelets
Piperidones
Pneumonia
Benzeneacetamides
Piperidones
Full Text Links
  • KJBT
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr