The Effect of Freezing-thawing Activated Platelet Rich Plasmas (PRP) on the Proliferations of Bacteria

Lim, Young Ae; Baik, SaeYun; Lee, Wee Gyo

Abstract

BACKGROUND
Fresh platelet rich plasma (PRP) gel has been reported to have anti-bacterial properties. We evaluated the anti-bacterial effects of liquid type activated PRP (tAPRP) using thrombin and heparin treatment after a freezing-thawing (F-T) procedure, using a disk diffusion method.
METHODS
PRP and platelet poor plasma (PPP) were prepared from CPDA-1 anticoagulated blood received from 20 donors. PRP was concentrated to 8 times the base platelet counts of donors for the first trial and to 11 times the base platelet counts of donors for the second trial. Both F-T PRP and F-T PPP were divided into a nonactivated group and an activated (tA) group, which was then treated with bovine thrombin and CaCl2, and heparin was added to prevent gel formation. The anti-bacterial effects of F-T PRP, F-T PPP, F-T tAPRP with heparin and F-T tAPPP with heparin on S. aureus and P. aeruginosa were evaluated using a disk-diffusion and direct dropping method. All experiments were duplicated.
RESULTS
The inhibited diameters resulting for S. aureus and P. aeruginosa, using the disk-diffusion and direct dropping method, were zero for all 20 sets of results for F-T PRP, F-T PPP, F-T tAPRP with heparin and F-T tAPPP with heparin.
CONCLUSION
No anti-bacterial effects were detected for S. aureus or P. aeruginosa in the F-T PRP, F-T PPP, F-T tAPRP with heparin and F-T tAPPP with heparin. This negative result may be due to the F-T treatment and/or because liquid instead of gel form of PRP was used. The use of the disk diffusion method for the determination of anti-bacterial ability of PRP may also be a factor in the negative study results.