Lab Anim Res.  2012 Sep;28(3):205-208. 10.5625/lar.2012.28.3.205.

Development of a type II diabetic mellitus animal model using Micropig(R)

Affiliations
  • 1Medikinetics Co., Ltd., Pyeong-Taek, Korea.
  • 2Center for Agricultural Biomaterials, Seoul National University, Seoul, Korea.
  • 3Qu-BEST Consulting Co., Ltd., Seongnam, Korea.
  • 4Laboratory of Developmental Genetics, College of Medicine, Inha Univerisity, Incheon, Korea. wklee@inha.ac.kr
  • 5Center for Advanced Medical Education by the BK21 Project, College of Medicine, Inha Univerisity, Incheon, Korea.

Abstract

Diabetes, which has shown an explosive increase in terms of its incidence, is regarded as a serious disease that must be overcome for the sake of human life. Among animal models used for testing of drug efficacy, the mini-pig model has shown a rapid upload due to its many similarities with human, particularly concerning the pharmacokinetics of compounds after subcutaneous administration, the structure and function of the gastrointestinal tract, the morphology of the pancreas, and overall metabolic status. Based on these various advantages, we sought to develop an animal model of type II diabetic mellitus using the Micro-pig, which differs from other miniature pigs. We used six male Micro-pigs for induction of a moderate insulin deficient model with nicotinamide (NIA)/streptozotocin (STZ) treatment and three animals for control. For evaluation of incidence of type II diabetes, we measured blood glucose level, and performed oral glucose tolerance test and immunohistochemistry on pancreatic tissue using insulin antibody. Compared to control animals, all animals treated with NIA/STZ showed high levels of glucose and low levels of insulin. In addition, we observed the partially destroyed beta cell population from tissue of the pancreas in treated animals. Based on these results, we report that the Micro-pig model developed in this study can be used for testing of the efficacy of therapeutic agents for treatment of Type 2 diabetic mellitus.

Keyword

Type 2 diabetic mellitus; Micropig; glucose level; NIA; STZ

MeSH Terms

Animals
Blood Glucose
Gastrointestinal Tract
Glucose
Glucose Tolerance Test
Humans
Immunohistochemistry
Incidence
Insulin
Male
Models, Animal
Niacinamide
Pancreas
Swine
Blood Glucose
Glucose
Insulin
Niacinamide

Figure

  • Figure 1 Blood glucose levels of fasting Micro-pigs was checked before feeding using Accu-Chek® Go. C: Control Micro-pig group (n=3). D: NIA/STZ-treated Micro-pig group (n=3).

  • Figure 2 Serum insulin levels of fasting Micro-pigs was checked before feeding using ELISA. C: Control Micro-pig group (n=1). D: NIA/STZ-treated Micro-pig group (n=3).

  • Figure 3 Immunohistochemistry (IHC) of pancreas with insulin antibody. A: HE staining of pancreas of control Micro-pig. B: IHC of pancreas of control Micro-pig with insulin Ab. C: HE staining of pancreas of NIA/STZ-treated Micro-pig. D: IHC of pancreas from NIA/STZ-treated Micro-pig with insulin Ab.


Cited by  2 articles

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Lab Anim Res. 2014;30(1):1-7.    doi: 10.5625/lar.2014.30.1.1.

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Jae Seung Jeong, Woon-Kyu Lee, Yeon Sung Moon, Na Rae Kim
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