Lab Anim Res.  2012 Sep;28(3):193-197. 10.5625/lar.2012.28.3.193.

Anticoccidial effects of Galla rhois extract on Eimeria tenella-infected chicken

• Lee HA
• Hong S
• Chung YH
• Song KD
• Kim O

• KMID: 1436724
• DOI: http://doi.org/10.5625/lar.2012.28.3.193

Abstract

Anticoccidial effects of Galla rhois (GR) extract were evaluated in chickens after oral infection with Eimeria tenella. This study was performed using 3-day-old chickens (n=30). The animals were divided into 3 groups as follows: GR 0.5%/infected (n=10), untreated/infected (n=10), and non-infected control (n=10). The chickens were fed a standard diet supplemented with or without GR for 1 week before infection with E. tenella (10,000 sporulated oocysts per chicken). The effects of GR on E. tenella infection were assessed by 2 parameters, number of fecal oocysts and body weight gain, and the results of the polymerase chain reaction (PCR). The GR-fed chickens produced significantly lower number of fecal oocysts (P<0.05) than the E. tenella-infected chickens who were fed the standard diet. In addition, GR-based diet improved the loss of body weight caused by E. tenella infection. Positive findings of PCR were identified by distinct bands in the samples of E. tenella-inoculated chickens. However, PCR analysis revealed no E. tenella oocysts in the feces of GR-fed chickens. Our data showed that GR extracts had remarkable anticoccidial activities against E. tenella. This finding might have implications for the development of novel anticoccidial drugs.

Keyword

Anticoccidial activity; coccidiosis; eimeria; eimeria tenella; galla rhois

MeSH Terms

Animals
Body Weight
Chickens
Coccidiosis
Diet
Eimeria
Eimeria tenella
Feces
Oocysts
Polymerase Chain Reaction

Figure

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  Figure 1 Amplification of the sample DNAs by species-specific polymerase chain reaction (PCR) for Eimeria tenella identified on a 2% agarose gel electrophoresis. 1~10: Animal numbers in the Group A, B, C. (A) DNAs extracted from the fecal samples of control chickens. (B) DNAs extracted from the fecal samples of E. tenella-infected chickens. (C) The DNAs extracted from the fecal samples of E. tenella-infected chickens treated with 0.5% Galla rhois.

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