Korean J Vet Res.  2012 Sep;52(3):177-181.

Serotype and antimicrobial susceptibility of Actinobacillus pleuropneumoniae isolates from pigs in Korea

• Jung JY
• Jang H

Abstract

Actinobacillus (A.) pleuropneumoniae is the causative agent of pleuropneumonia which is one of the most important respiratory diseases in pigs worldwide. A total of 32 A. pleuropneumoniae isolates from diseased pigs during 2008 to 2010 were serotyped by polymerase chain reaction method. The susceptibility of the isolates to 13 antimicrobial agents were determined by disk diffusion test. In all the 32 isolates examined in this study, serotype 5 (16 isolates: 50%), 1 (7 isolates: 21.9%), 2 (5 isolates: 15.6%) and 12 (1 isolate: 3.1%) were found. Of all tested antimicrobial agents, resistance to oxytetracycline was found in 96.9% of isolates, followed by resistance to amikacin (81.2%), neomycin (68.7%), kanamycin (53.1%), penicillin (50.0%), gentamicin (43.7%), florfenicol (25.0%), ampicillin (18.7%), colistin (9.4%), trimethoprim/sulfamethoxazole, ceftiofur (8.3%), amoxicillin/clavulanic acid (3.1%) and enrofloxacin (0%). Oxytetracycline or florfenicol-resistant isolates were examined for the presence of resistance gene. Among the 31 oxytetracycline-resistant isolates, tetB, tetH and tetO genes were detected in 22 (71%), 8 (26%) and 1 (3%) isolates, respectively. The floR genes were detected in 8 (100%) of the 8 florfenicol-resistant A. pleuropneumoniae isolates.

Keyword

Actinobacillus pleuropneumoniae; antimicrobial susceptibility; pig; resistance gene; serotype

MeSH Terms

Actinobacillus
Actinobacillus pleuropneumoniae
Amikacin
Ampicillin
Anti-Infective Agents
Cephalosporins
Colistin
Diffusion
Fluoroquinolones
Gentamicins
Kanamycin
Korea
Neomycin
Oxytetracycline
Penicillins
Pleuropneumonia
Polymerase Chain Reaction
Swine
Thiamphenicol
Amikacin
Ampicillin
Anti-Infective Agents
Cephalosporins
Colistin
Fluoroquinolones
Gentamicins
Kanamycin
Neomycin
Oxytetracycline
Penicillins
Thiamphenicol