Korean J Orthod.  2012 Oct;42(5):263-269. 10.4041/kjod.2012.42.5.263.

Antimicrobial effect of different brushing frequencies with fluoride toothpaste on Streptococcus mutans and Lactobacillus species in children with fixed orthodontic appliances

Affiliations
  • 1Department of Pharmacology, School of Dental Medicine, University of Zagreb, Zagreb, Croatia. peros@sfzg.hr
  • 2Department of Orthodontics, School of Dental Medicine, University of Zagreb, Zagreb, Croatia.

Abstract


OBJECTIVE
To evaluate the antimicrobial effect of different frequencies of brushing with fluoride toothpaste on the levels of salivary mutans streptococci and lactobacilli in children undergoing fixed orthodontic treatment.
METHODS
The study included 22 patients scheduled for fixed orthodontic therapy distributed between 2 groups with different hygiene regimes. All the subjects received identical braces, bands, and brackets bonded with the same material. Stimulated saliva samples were obtained before placement of the appliance and at 6, 12, and 18 weeks during the therapy. Saliva samples were cultured on selective microbial agar for the detection of microorganisms.
RESULTS
Salivary mutans streptococci were significantly suppressed throughout the experimental period in the group that brushed 4 times a day as compared to the group that brushed twice a day. Salivary lactobacilli were not significantly affected by the frequency of brushing with 0.32% sodium fluoride (NaF) toothpaste.
CONCLUSIONS
The use of 0.32% NaF-containing toothpaste more than 3 times a day has effective antimicrobial activity on mutans streptococci but not lactobacilli in the saliva of children with fixed orthodontic appliances.

Keyword

Microbiology; Oral hygiene

MeSH Terms

Agar
Braces
Child
Fluorides
Humans
Hygiene
Lactobacillus
Oral Hygiene
Orthodontic Appliances
Saliva
Sodium Fluoride
Streptococcus
Streptococcus mutans
Toothpastes
Agar
Fluorides
Sodium Fluoride
Toothpastes

Reference

1. Huser MC, Baehni PC, Lang R. Effects of orthodontic bands on microbiologic and clinical parameters. Am J Orthod Dentofacial Orthop. 1990. 97:213–218.
Article
2. Rosenbloom RG, Tinanoff N. Salivary Streptococcus mutans levels in patients before, during, and after orthodontic treatment. Am J Orthod Dentofacial Orthop. 1991. 100:35–37.
Article
3. Loesche WJ. Role of Streptococcus mutans in human dental decay. Microbiol Rev. 1986. 50:353–380.
Article
4. Kneist S, Schmidt F, Callaway A, Willershausen B, Rupf S, Wicht M, et al. Diversity of Lactobacillus species in deep carious lesions of primary molars. Eur Arch Paediatr Dent. 2010. 11:181–186.
Article
5. Kupietzky A, Majumdar AK, Shey Z, Binder R, Matheson PB. Colony forming unit levels of salivary Lactobacilli and Streptococcus mutans in orthodontic patients. J Clin Pediatr Dent. 2005. 30:51–53.
Article
6. Attin R, Ilse A, Werner C, Wiegand A, Attin T. Antimicrobial effectiveness of a highly concentrated chlorhexidine varnish treatment in teenagers with fixed orthodontic appliances. Angle Orthod. 2006. 76:1022–1027.
Article
7. Section on Pediatric Dentistry and Oral Health. Preventive oral health intervention for pediatricians. Pediatrics. 2008. 122:1387–1394.
8. Benson PE, Parkin N, Millett DT, Dyer FE, Vine S, Shah A. Fluorides for the prevention of white spots on teeth during fixed brace treatment. Cochrane Database Syst Rev. 2004. (3):CD003809.
Article
9. Derks A, Katsaros C, Frencken JE, van't Hof MA, Kuijpers-Jagtman AM. Caries-inhibiting effect of preventive measures during orthodontic treatment with fixed appliances. A systematic review. Caries Res. 2004. 38:413–420.
Article
10. Buzalaf MA, Pessan JP, Honório HM, ten Cate JM. Mechanisms of action of fluoride for caries control. Monogr Oral Sci. 2011. 22:97–114.
Article
11. Koo H. Strategies to enhance the biological effects of fluoride on dental biofilms. Adv Dent Res. 2008. 20:17–21.
Article
12. Marquis RE, Clock SA, Mota-meira M. Fluoride and organic weak acids as modulators of microbial physiology. FEMS Microbiol Rev. 2003. 26:493–510.
Article
13. Rosin-Grget K, Sutej I, Lincir I. The effect of saliva on the formation of KOH-soluble fluoride after topical application of amine fluoride solutions of varying fluoride concentration and pH. Caries Res. 2007. 41:235–238.
Article
14. Chang HS, Walsh LJ, Freer TJ. The effect of orthodontic treatment on salivary flow, pH, buffer capacity, and levels of mutans streptococci and lactobacilli. Aust Orthod J. 1999. 15:229–234.
15. Walsh T, Worthington HV, Glenny AM, Appelbe P, Marinho VC, Shi X. Fluoride toothpastes of different concentrations for preventing dental caries in children and adolescents. Cochrane Database Syst Rev. 2010. (1):CD007868.
Article
16. Dénes J, Gábris K. Results of a 3-year oral hygiene programme, including amine fluoride products, in patients treated with fixed orthodontic appliances. Eur J Orthod. 1991. 13:129–133.
Article
17. Kanaya T, Kaneko N, Amaike C, Fukushima M, Morita S, Miyazaki H, et al. The effect of orthodontic appliances on levels of Streptococcus mutans, Streptococcus sobrinus and microbial flora in saliva. Int Congr Ser. 2005. 1284:189–190.
Article
18. Boersma JG, van der Veen MH, Lagerweij MD, Bokhout B, Prahl-Andersen B. Caries prevalence measured with QLF after treatment with fixed orthodontic appliances: influencing factors. Caries Res. 2005. 39:41–47.
Article
19. Alexander SA, Ripa LW. Effects of self-applied topical fluoride preparations in orthodontic patients. Angle Orthod. 2000. 70:424–430.
20. Vierrou AM, Manwell MA, Zamek RL, Sachdeva RC, Tinanoff N. Control of Streptococcus mutans with topical fluoride in patients undergoing orthodontic treatment. J Am Dent Assoc. 1986. 113:644–646.
Article
21. Dikeman TL. A study of acidogenic and aciduric microorganisms in orthodontic and non-orthodontic patients. Am J Orthod. 1962. 48:627–628.
Article
22. Jenatschke F, Elsenberger E, Welte HD, Schlagenhauf U. Influence of repeated chlorhexidine varnish applications on mutans streptococci counts and caries increment in patients treated with fixed orthodontic appliances. J Orofac Orthop. 2001. 62:36–45.
Article
23. Lundström F, Krasse B. Caries incidence in orthodontic patients with high levels of Streptococcus mutans. Eur J Orthod. 1987. 9:117–121.
Article
24. Madléna M, Vitalyos G, Márton S, Nagy G. Effect of chlorhexidine varnish on bacterial levels in plaque and saliva during orthodontic treatment. J Clin Dent. 2000. 11:42–46.
25. Geiger AM, Gorelick L, Gwinnett AJ, Benson BJ. Reducing white spot lesions in orthodontic populations with fluoride rinsing. Am J Orthod Dentofacial Orthop. 1992. 101:403–407.
Article
26. Marinho VC, Higgins JP, Sheiham A, Logan S. One topical fluoride (toothpastes, or mouthrinses, or gels, or varnishes) versus another for preventing dental caries in children and adolescents. Cochrane Database Syst Rev. 2004. (1):CD002780.
Article
27. Lara-Carrillo E, Montiel-Bastida NM, Sánchez-Pérez L, Alanís-Tavira J. Changes in the oral environment during four stages of orthodontic treatment. Korean J Orthod. 2010. 40:95–105.
Article
28. Marinho VC. Cochrane reviews of randomized trials of fluoride therapies for preventing dental caries. Eur Arch Paediatr Dent. 2009. 10:183–191.
Article
29. Nordström A, Birkhed D. Effect of a third application of toothpastes (1450 and 5000 ppm F), including a 'massage' method on fluoride retention and pH drop in plaque. Acta Odontol Scand. 2012. [Epub ahead of print].
Article
30. Kaneko N, Yoshihara A, Ida H, Nomura Y, Imai S, Nisizawa T, et al. Influence of a fluoride mouthrinse on mutans streptococci in schoolchildren. Caries Res. 2006. 40:501–507.
Article
31. del Carmen Ahumada Ostengo M, Wiese B, Nader-Macias ME. Inhibitory effect of sodium fluoride and chlorhexidine on the growth of oral lactobacilli. Can J Microbiol. 2005. 51:133–140.
Article
32. Bradshaw DJ, Mckee AS, Marsh PD. Prevention of population shifts in oral microbial communities in vitro by low fluoride concentrations. J Dent Res. 1990. 69:436–441.
Article
Full Text Links
  • KJOD
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr