J Bacteriol Virol.  2012 Mar;42(1):41-47. 10.4167/jbv.2012.42.1.41.

Production of the Monoclonal Antibodies against Bartonella henselae Isolated from a Korean Patient

• Kil SH
• Kang JS

• KMID: 1434771
• DOI: http://doi.org/10.4167/jbv.2012.42.1.41

Abstract

Bartonellosis is spotlighted recently as an emerging zoonosis and Bartonella henselae is reported to be the main infectious agent. In Korea, however, few studies have been made on the epidemiology and microbiology on bartonellosis. Thus, this study was conducted to produce a new monoclonal antibody that can be used for identifying B. henselae. In order to prepare monoclonal antibodies against B. henselae, we inoculated mice with the isolated strain from Korean patient and performed cell fusion experiment. The selected hybridoma clones produced monoclonal antibodies which showed positive immunofluorescence staining of bacteria and specific protein bands in western blot analysis. In order to examine whether these antibodies could be used for the identifying and quantifying Bartonella, we performed confocal microscopy and flow cytometry using the new antibodies. These monoclonal antibodies can be used as a useful tool in further researches on the biology of Bartonella.

Keyword

Bartonella henselae; Bartonellosis; Monoclonal antibody

MeSH Terms

Animals
Antibodies
Antibodies, Monoclonal
Bacteria
Bartonella
Bartonella henselae
Bartonella Infections
Biology
Blotting, Western
Cell Fusion
Clone Cells
Flow Cytometry
Fluorescent Antibody Technique
Humans
Hybridomas
Korea
Mice
Microscopy, Confocal
Sprains and Strains
Antibodies
Antibodies, Monoclonal