J Korean Med Sci.  1986 Sep;1(1):5-13. 10.3346/jkms.1986.1.1.5.

Anti-HBc IgM and anti-delta screening by EIA method

• Kim JS
• Kim JM

• KMID: 1421229
• DOI: http://doi.org/10.3346/jkms.1986.1.1.5

Abstract

The clinical value of an enzyme-linked immunosorbent assay (ELISA) for the detection of anti-HBc IgM was evaluated by testing 202 sera from acute viral hepatitis B (AVHB), hepatitis B (HB), chronic hepatitis (CAH), chronic liver disease (CLD), cirrhosis, primary hepatoma, HBsAg carrier, acute viral hepatitis A (AVHA), hepatitis A (HA), non-A, non-B (NANB) hepatitis and miscellaneous conditions other than hepatic disease, and 19 additional various hepatic disease cases were examined for anti-delta. In clinical situations the accurate diagnosis of HB is not always possible and the differential diagnosis seems to be very important especially in making decisions of treatment and estimation of prognosis. In overall cases the highest positive rate of anti-HBc IgM was found in AVHB as shown as 74.3% (26/35) comparing to other conditions in which the positive rate was extremely low (2.1%). The anti-HBc IgM appeared to be highly specific to AVHB (83.9%) as compared to the other. The positive rate of HBsAg was high in AVHB, CAH and HBsAg carrier (100.0%) followed by CLD, cirrhosis and HB (up to 70.8%). The ALT activities and ALPalb fractions were significantly high in AVHB (p less than 0.005). The correlation between the positivity of anti-HBc IgM and highly abnormal ALT appeared be high. AVHB was confined mostly to 10-20 age group and the male to female ratio was about 6 to 1. Subgroup of AVHB II with positive anti-HBc IgM appeared to have a greater chance being positive for HBsAg and ALPalb. The S/N ratio of anti-HBc IgM was as high as 20 which was unique to AVHB.

Keyword

Anti-HBc igM; anti-HBc; HBeAg; anti-HBs; HBe Ag; anti-HBe; anti-delta; HBV DNA; HBV DNA polymerase; S/N ratio

MeSH Terms

Adolescent
Adult
Biological Markers
Child
Diagnosis, Differential
Female
Hepatitis/*diagnosis
Hepatitis Antibodies/*analysis
Hepatitis B/diagnosis/immunology
Hepatitis B Antibodies/analysis
Hepatitis Delta Virus/*immunology
Humans
*Immunoenzyme Techniques
Immunoglobulin M/immunology
Isoenzymes/immunology
Male
Middle Aged
Biological Markers
Hepatitis Antibodies
Hepatitis B Antibodies
Immunoglobulin M
Isoenzymes