Exp Mol Med.  2004 Apr;36(2):135-144.

Trp-Lys-Tyr-Met-Val-Met stimulates phagocytosis via phospho-lipase D-dependent signaling in mouse dendritic cells

• Lee HY
• Kang HK
• Jo EJ
• Kim JI
• Lee YN
• Lee SH
• Park YM
• Ryu SH
• Kwak JY
• Bae YS

Abstract

Dendritic cells (DCs) play a key role in activating the immune response against invading pathogens as well as dying cells or tumors. Although the immune response can be initiated by the phagocytic activity by DCs, the molecular mechanism involved in this process has not been fully investigated. Trp-Lys-Tyr-Met-Val-Met-NH2 (WKYMVM) stimulates the activation of phospholipase D (PLD) via Ca2+ increase and protein kinase C activation in mouse DC cell line, DC2.4. WKYMVM stimulates the phagocytic activity, which is inhibited in the presence of N-butanol but not t-butanol in DC2.4 cells. Furthermore, the addition of phosphatidic acid, an enzymatic product of PLD activity, enhanced the phagocytic activity in DC2.4 cells. Since at least two of formyl peptide receptor (FPR) family (FPR1 and FPR2) are expressed in DC2.4 as well as in mouse bone marrow-derived dendritic cells, this study suggests that the activation of FPR family by WKYMVM stimulates the PLD activity resulting in phagocytic activity in DC2.4 cells.

Keyword

chemotactic factors; dendritic cell; N-formylmethionine leucyl-phenylalamine; oligopeptides; phagocytosis; phospholipase D; receptors; formyl peptid

MeSH Terms

1-Butanol/pharmacology
Animals
Bone Marrow Cells/cytology/metabolism
Calcium Signaling/*drug effects
Cell Death/immunology
Cell Line
Communicable Diseases/immunology
Dendritic Cells/immunology/*metabolism
Mice
Neoplasms/immunology
Oligopeptides/*pharmacology
Phagocytosis/*drug effects
Phosphatidic Acids/pharmacology
Phospholipase D/*metabolism
Receptors, Formyl Peptide/*metabolism
Research Support, Non-U.S. Gov't
tert-Butyl Alcohol/pharmacology