J Vet Sci.  2012 Jun;13(2):211-213. 10.4142/jvs.2012.13.2.211.

SRY-positive 78, XY ovotesticular disorder of sex development in a wolf cloned by nuclear transfer

• Kang JT
• Kim HJ
• Oh HJ
• Hong SG
• Park JE
• Kim MJ
• Kim MK
• Jang G
• Kim DY
• Lee BC

• KMID: 1376198
• DOI: http://doi.org/10.4142/jvs.2012.13.2.211

Abstract

Recently, we reported the three wolves cloning with normal karyotype from somatic cells of endangered male gray wolves (Canis lupus), but one wolf had female external genitalia. In this study, we conducted further clinical, histological, and genetic analyses. This cloned wolf had a normal uterus but developed ovotestis. Through molecular analysis of the SRY gene, a mutation in the coding sequence of SRY gene could be excluded as a cause of intersexuality. This is the first report of a cloned wolf with a 78, XY ovotesticular disorder affecting sexual development characterized by bilateral ovotestes.

Keyword

hermaphrodite; intersexuality; wolf

MeSH Terms

Animals
Cloning, Organism/*veterinary
Female
Karyotyping
Mutation
Nuclear Transfer Techniques/*veterinary
Ovotesticular Disorders of Sex Development/pathology/*veterinary
*Wolves

Figure

• 

  Fig. 1 SRY-positive 78, XY ovotesticular disorder of sex development in a cloned gray wolf. (A) Image of the ovary-like and uterus structures. (B) Histopathology of the ovotestis in the wolf. Note the hypoplastic seminiferous tubules (arrows) and undifferentiated primitive ovarian follicles (arrowheads). H&E stain. Scale bar = 100 µm. (C) Karyotype determined from skin fibroblasts collected for chromosome analysis. No chromosomal abnormalities were observed.

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