Exp Mol Med.  2009 Aug;41(8):548-554. 10.3858/emm.2009.41.8.060.

Cordycepin inhibits UVB-induced matrix metalloproteinase expression by suppressing the NF-kappa B pathway in human dermal fibroblasts

Affiliations
  • 1Department of Biochemistry and Institute for Medical Sciences, Chonbuk National University Medical School, Jeonju 560-182, Korea. jsukim@chonbuk.ac.kr
  • 2Department of Dermatology, Chonbuk National University Medical School, Jeonju 560-182, Korea.
  • 3Department of Obstetrics and Gynecology, Chonbuk National University Medical School, Jeonju 560-182, Korea.
  • 4Department of Physiology, School of Oriental Medicine, Wonkwang University, Iksan 570-749, Korea.
  • 5Department of Companion and Laboratory Animal Science, Kongju National University, Yesan 340-702, Korea.
  • 6Division of Animal Science and Resources Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 305-764, Korea.

Abstract

Cordycepin (3'-deoxyadenosine) has been shown to exhibit many pharmacological activities, including anti-cancer, anti-inflammatory, and anti-infection activities. However, the anti-skin photoaging effects of cordycepin have not yet been reported. In the present study, we investigated the inhibitory effects of cordycepin on matrix metalloproteinase-1 (MMP-1) and -3 expressions of the human dermal fibroblast cells. Western blot analysis and real-time PCR revealed cordycepin inhibited UVB-induced MMP-1 and -3 expressions in a dose-dependent manner. UVB strongly activated NF-kappa B activity, which was determined by I kappa B alpha degradation, nuclear localization of p50 and p65 subunit, and NF-kappa B binding activity. However, UVB-induced NF-kappa B activation and MMP expression were completely blocked by cordycepin pretreatment. These findings suggest that cordycepin could prevent UVB-induced MMPs expressions through inhibition of NF-kappa B activation. In conclusion, cordycepin might be used as a potential agent for the prevention and treatment of skin photoaging.

Keyword

cordycepin; matrix metalloproteinases; NF-kappa B; skin aging; ultraviolet rays

MeSH Terms

Aging/physiology
Cells, Cultured
Deoxyadenosines/*pharmacology
*Dermis/cytology/drug effects/physiology/radiation effects
Dose-Response Relationship, Drug
Enzyme Induction/drug effects
Fibroblasts/drug effects/metabolism/radiation effects
Gene Expression Regulation, Enzymologic
Humans
Infant, Newborn
Male
*Matrix Metalloproteinase 1/antagonists & inhibitors/biosynthesis/genetics/radiation effects
Matrix Metalloproteinase 3/antagonists & inhibitors/*biosynthesis/genetics/radiation effects
NF-kappa B/*antagonists & inhibitors/genetics/metabolism
Skin/physiopathology/radiation effects
*Ultraviolet Rays
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