J Vet Sci.
2001 Dec;2(3):159-166.
Cloning of canine GM-CSF and SCF genes
- Affiliations
-
- 1Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
- 2Cancer Research Division, National Institute of Health, Seoul 122-701, Korea.
- 3Department of Veterinary Internal Medicine, Tokyo University of Agriculture and Technology, 3-5-8, Saiwai-cho, Fuchu, Tokyo 183-8509, Japan
Abstract
- Cytokines have pleiotropic regulatory effects on hematopoietic cells and many other cell types that participate in host defence and repair processes. Granulocyte-macrophage colony-stimulating factor (GM-CSF) mediates the growth and differentiation of granulocytes and macrophages and regulates the biological functions expressed by mature cells of these lineages. Stem cell factor (SCF) is a multifunctional cytokine involved in hematopoiesis, melanogenesis and gametogenesis. In order to determine the complementary DNA (cDNA) of canine GM-CSF and canine SCF, cDNA clones were generated from lipopolysaccharide (LPS) stimulated peripheral blood mononuclear cells (PBMCs) and bone marrow cells by reverse transcription PCR amplification. The canine GM-CSF cDNA obtained in this study contains an open reading frame encoding 144 amino acid residues and has 53-75% homology with those of human, cat, sheep, pig, cow and mouse, Canine SCF cDNA consist of an open reading frame encoding 274 amino acid residues and shares 81-92% homology with those of human, cat, pig, cow and mouse.
MeSH Terms
-
Amino Acid Sequence
Animals
Base Sequence
Cats
Cattle
*Cloning, Molecular
Codon
DNA, Complementary/analysis/*genetics
Dogs/blood/*genetics
Gametogenesis
Gene Amplification
Granulocyte-Macrophage Colony-Stimulating Factor/chemistry/*genetics
Hematopoiesis
Humans
Mice
Molecular Sequence Data
Open Reading Frames
Reverse Transcriptase Polymerase Chain Reaction/methods
Sequence Alignment
Sequence Homology, Amino Acid
Sheep
Stem Cell Factor/chemistry/*genetics
Swine
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