J Vet Sci.  2005 Jun;6(2):103-109.

Genotoxicity and toxicological effects of acrylamide on reproductive system in male rats

Affiliations
  • 1Department of Biochemistry, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. vetlee@snu.ac.kr
  • 2Brain Korea 21, School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea.

Abstract

The toxicity of acrylamide was evaluated through mutagenicity of Salmonella, chromosome aberration of Chinese hamster lung fibroblasts, micronucleus formation in mice and reproductive toxicity in rats. Based on Ames test, acrylamide showed mutagenic potency for strains TA98 and TA100. Moreover, both chromosomal aberration assay and micronucleus assay indicated that acrylamide might have genotoxic potency; the chromosomal aberration frequencies were observed to be proportional to acrylamide concentrations of 5-50 mM, and acrylamide significantly increased micronuclei in peripheral blood cells of mice at doses of higher than 72.5 mg/kg. Male rats were treated with acrylamide at doses of 0, 5, 15, 30, 45, or 60 mg/kg/day for 5 consecutive days, and the toxicity of acrylamide was observed. In the group treated with the highest dose of acrylamide (60 mg/kg/day), the loss of body weight and reduced testis weight were observed. Also the epididymides weights were reduced significantly in all the groups treated with acrylamide. The number of sperms in cauda epididymidis decreased significantly in an acrylamide dose-dependent manner. Rats treated with 60 mg/kg/day of acrylamide showed several histopathological lesions in the seminiferous tubules. There were thickening and multiple layering of the tubular endothelium, and the formation of many multinucleated giant cells in seminiferous tubules. Taken together, acrylamide not only causes the genotoxicity of eukaryotic cells and mice but also shows the toxicological effects on reproductive system in male rats.

Keyword

acrylamide; chromosomal aberration; genotoxicity; micronuclei; mutagenicity

MeSH Terms

Acrylamide/*toxicity
Animals
Body Weight
Carcinogens/*toxicity
Chromosome Aberrations/chemically induced
Cricetinae
Cricetulus
Epididymis/*drug effects/pathology
Histocytochemistry
Male
Mice
Mice, Inbred ICR
Micronucleus Tests
Mutagenicity Tests
Organ Size
Rats
Rats, Sprague-Dawley
Seminiferous Tubules/*drug effects/pathology
Sperm Count
Full Text Links
  • JVS
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr