J Vet Sci.  2007 Dec;8(4):401-407. 10.4142/jvs.2007.8.4.401.

Sequence analysis of the S1 glycoprotein gene of infectious bronchitis viruses: identification of a novel phylogenetic group in Korea

Affiliations
  • 1School of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 200-701, Korea. kwonhm@kangwon.ac.kr
  • 2College of Veterinary Medicine, Konkuk University, Seoul 143-701, Korea.

Abstract

Twelve Korean infectious bronchitis viruses (IBVs) were isolated in the field from chickens suspected of being carriers of infectious bronchitis between 2001 and 2003. The S1 glycoprotein genes of these IBV isolates were amplified by reverse transcriptase-polymerase chain reaction (RTPCR) and analyzed by restriction fragment length polymorphism (RFLP) analysis. These Korean IBV isolates were classified into three groups according to their RFLP patterns obtained using the restriction enzyme HaeIII. Half of the twelve isolates were similar to the KM91 RFLP pattern, which is a common pattern in Korea. Three more isolates were related to the Arkansas strain pattern, but with some unique variations. The other three viruses showed variant RFLP patterns. For a comparison with the published sequences for non-Korean IBV strains, amplified PCR products from the twelve isolates were cloned and sequenced. The Korean IBV field isolates had 71.2-99.7% nucleotide sequence homology with each other and 45.9-80.7% nucleotide sequence homology with non-Korean IBV strains. With respect to the deduced amino acid sequence, the Korean IBV isolates had 71.5-99.3% similarity with each other and 44.9-80.3% similarity with non-Korean IBV strains. Phylogenetic tree analysis revealed that some of the IBV isolates appear to belong to a new group, different from the non-Korean IBV strains or from previously isolated Korean IBV strains. Specifically, the new Korean IBV isolates K10217-03, K3-3 and K1255-03 represented a separate group. These findings suggest that the Korean IBVs appear to be continuously evolving.

Keyword

infectious bronchitis virus; phylogenetic tree; S1 glycoprotein gene; sequencing

MeSH Terms

Amino Acid Sequence
Animals
Coronavirus Infections/*veterinary/virology
Glycoproteins/chemistry/*genetics
Infectious bronchitis virus/*classification/*genetics/isolation & purification
Molecular Sequence Data
*Phylogeny
Polymorphism, Restriction Fragment Length
Poultry
Poultry Diseases/*virology
Reverse Transcriptase Polymerase Chain Reaction/veterinary
Sequence Alignment/veterinary
Sequence Analysis
Sequence Homology, Amino Acid
Viral Proteins/chemistry/genetics

Figure

  • Fig. 1 RFLP patterns of the PCR-amplified S1 glycoprotein genes from representative Korean IBV isolates digested with restriction enzyme Hae III. Lanes 1: 100 bp plus DNA ladder; Lane 2: K044-02; Lane 3: K434-01; Lane 4: K 3-3; Lane 5: K2-6; Lane 6: K545-02.

  • Fig. 2 The deduced amino acid sequences of the S1 glycoprotein gene of 13 Korean IBV isolates and six published non-Korean IBV strains. The dashes (-) indicate regions where the sequences are identical to those of K748-01. Deletions within the sequences are shown with asterisks (*).

  • Fig. 3 Phylogenetic relationship based on the deduced amino acid sequences of the S1 glycoprotein of the 12 Korean IBV field isolates (K434-01, K748-01, K058-02, K044-02, K117-02, K234-02, K545-02, K514-03, K10217-03, K1255-03, K3-3, K3-3) and non-Korean IBV strains generated by maximum parsimony method with heuristic search and 100 bootstrap replicates. The length of each branch represents the number of amino acid changes between sequences.


Cited by  1 articles

Pathogenicity and antigenicity of a new variant of Korean nephropathogenic infectious bronchitis virus
Kang-Seuk Choi, Eun-Kyoung Lee, Woo-Jin Jeon, Mi-Ja Park, Jin-Won Kim, Jun-Hun Kwon
J Vet Sci. 2009;10(4):357-359.    doi: 10.4142/jvs.2009.10.4.357.


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