J Vet Sci.  2008 Dec;9(4):345-349. 10.4142/jvs.2008.9.4.345.

The expression and localization of inhibin isotypes in mouse testis during postnatal development

Affiliations
  • 1Department of Veterinary Anatomy, College of Veterinary Medicine and Veterinary Medical Research Center, Chonnam National University, Gwangju 500-757, Korea. moonc@chonnam.ac.kr
  • 2Department of Veterinary Toxicology, College of Veterinary Medicine and Veterinary Medical Research Center, Chonnam National University, Gwangju 500-757, Korea.
  • 3Department of Veterinary Surgery, College of Veterinary Medicine and Veterinary Medical Research Center, Chonnam National University, Gwangju 500-757, Korea.
  • 4Department of Veterinary Obstetrics, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea.
  • 5Department of Veterinary Anatomy, College of Veterinary Medicine and Applied Radiological Science Research Institute, Cheju National University, Jeju 690-756, Korea.

Abstract

Inhibin, which is important for normal gonadal function, acts on the pituitary gonadotropins to suppress folliclestimulating hormone (FSH) secretion. The level and cellular localization of the inhibin isotypes, alpha, beta(A) and beta(B), in the testis of mice were examined during postnatal development in order to determine if inhibin expression is related to testicular maturation. Mouse testes were sampled on postnatal days (PNDs) 1, 3, 6, 18, 48 and 120, and analyzed by Western blotting and immunofluorescence. Western blot analysis showed very low levels of inhibin alpha, beta(A) and beta(B) expression in the testes at days 1 to 6 after birth. The levels then increased gradually from PND 18 to 48-120, and there were significant peaks at PND 48. Inhibin alpha, beta(A) and beta(B) were detected in testicular cells during postnatal development using immunohistochemistry. The immunoreactivity of inhibin alpha was rarely observed in testicular cells during PND 1 to 6, or in the cytoplasmic process of Sertoli cells surrounding the germ cells and interstitial cells during PND 18 to 120. Inhibin beta(A) and beta(B) immunoreactivity was rarely observed in the testis from PND 1 to 6. On the other hand, it was observed in some spermatogonial cells, as well as in the interstitial space between PND 48 and PND 120. We conclude that the expression of inhibin isotypes increases progressively in the testis of mice with increasing postnatal age, suggesting that inhibin is associated with a negative feedback signal for FSH in testicular maturation.

Keyword

inhibin; mouse; postnatal development; Sertoli cell; testis
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