J Vet Sci.  2006 Mar;7(1):53-58. 10.4142/jvs.2006.7.1.53.

Isolation and characterization of Streptococcus sp. from diseased flounder (Paralichthys olivaceus) in Jeju Island

Affiliations
  • 1Department of Oceanography, Pukyong National University, Busan 608-737, Korea.
  • 2College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. parksec@snu.ac.kr
  • 3College of Fisheries and Aquatic Sciences, Iloilo State College of Fisheries, Iloilo, Philippines.
  • 4KRF Zoonotic Disease Priority Research Institute, Seoul National University, Seoul 151-742, Korea.

Abstract

Streptococcus sp. is gram-positive coccus that causes streptococcal infections in fish due to intensification of aquaculture and caused significant economic losses in fish farm industry. A streptococcal infection occurred from cultured diseased olive flounder (Paralichthys olivaceus) in May, 2005 at a fish farm in Jeju Island, Korea. The diseased flounder exhibited bilateral exophthalmic eyes and rotten gills; water temperature was 16~18oC when samples were collected. Of the 22 fish samples collected, 3 samples were identified as Lactococcus garvieae and 18 samples were identified as Streptococcus parauberis by culture-based, biochemical test. Serological methods such as slide agglutination, hemolysis and antimicrobial susceptibility test were also used as well as multiplex PCRbased method to simultaneously detect and confirm the pathogens involved in the infection. S. parauberis and L. garvieae have a target region of 700 and 1100 bp., respectively. One fish sample was not identified because of the difference in the different biochemical and serological tests and was negative in PCR assay. In the present study, it showed that S. parauberis was the dominant species that caused streptococcosis in the cultured diseased flounder.

Keyword

Lactococcus garvieae; multiplex PCR assay; Streptococcus parauberis; Streptococcus sp.

MeSH Terms

Agglutination Tests/veterinary
Animals
Aquaculture
DNA, Bacterial/chemistry/genetics
Fish Diseases/*microbiology
*Flounder
Hemolysis
Korea
Microbial Sensitivity Tests/veterinary
Polymerase Chain Reaction/veterinary
Streptococcal Infections/microbiology/*veterinary
Streptococcus/genetics/*isolation&purification

Figure

  • Fig. 1 Representative amplification products obtained using the multiplex PCR assay for detection of streptococcus species in flounder fish. Lanes M, 100-bp DNA ladder; lanes P, positive control (S. iniae 0404M, 300 bp); lane N, negative control; lane 1, negative for streptococcal infection; lanes 2 and 3, positive for L. garviae (1,100 bp); lanes 4~9, positive for S. parauberis (718 bp).


Reference

1. Al-Harbi , Ahmed H. First Isolation of Streptococcus sp. from hybrid tilapia (Orcheochromis niloticus x O. aureus) in Saudi Arabia. Aquaculture. 1994. 128:195–201.
Article
2. Austin B, Austin DA, editors. Diseases of Farmed and Wild Fish. 1990. Berlin: Springer-Verlag;13–15.
3. Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966. 45:493–496.
Article
4. Baya AM, Lupiani B, Hetrick FM, Robertson BS, Lukacovic R, May E, Poukish C. Association of Streptococcus sp. with mortalities in the Chesapeake Bay and its tributaries. J Fish Dis. 1990. 13:251–253.
Article
5. Bercovier H, Ghittino C, Eldar A. Immunization with bacterial antigens: infectious with streptococci and related organism. Dev Biol Stand. 1997. 90:153–160.
6. Berridge BR, Bercovier H, Frelier PF. Streptococcus agalactiae and Streptococcus difficile 16S-23S intergenic rDNA: genetic homogeneity and species-specific PCR. Vet Microbiol. 2001. 78:165–173.
Article
7. Chang PH, Plumb JA. Histopathology of experimental Streptococcus sp. infection in Tilapia, Oreochromis niloticus (L.), and channel catfish, Ictalurua punctatus (Rafinesque). J Fish Dis. 1996. 19:235–241.
Article
8. Chen SC, Liaw LL, Su HY, Ko SC, Wu CY, Chang HC, Tsai YH, Yang KL, Chen YC, Chen TH, Lin GR, Cheng SY, Lin YD, Lee JL, Lai CC, Weng YJ, Chu SY. Lactococcus garvieae, a cause of disease in grey mullet, Mugil cephalus L., in Taiwan. J Fish Dis. 2002. 25:727–732.
Article
9. Collins MD, Farrow JAE, Phillips BA, Kandler O. Streptococcus garvieae sp. nov. and Streptococcus plantarum sp. nov. J Gen Microbiol. 1984. 129:3427–3431.
10. Domenech a, Fernandez-Garayzabal JF, Pascual C, Garcia JA, Cutuli MT, Moreno MA, Collins MD, Dominguez L. Streptococcosis in cultured turbot, Scopthalmus maximus (L.), associated with Streptococcus parauberis. J Fish Dis. 1996. 19:33–38.
11. Eldar A, Bejerano Y, Bercovier H. Streptococcus shiloi and Streptococcus difficile: two new streptococcal species causing a meningoencephalitis in fish. Curr Microbiol. 1994. 28:139–143.
Article
12. Eldar A, Frelier PF, Assenta L, Varner PW, Lawhon S, Bercovier H. Streptococcus shiloi, the name for an agent causing septicemic infection in fish, is a junior synonym of Streptococcus iniae. Int J Syst Bacteriol. 1995. 45:840–842.
Article
13. Eldar A, Ghittino C. Lactococcus garvieae and Streptococcus iniae infections in rainbow trout, Oncorhynchus mykiss: similar but different diseases. Dis Aquat Org. 1999. 36:227–231.
Article
14. Eldar A, Ghittino C, Asanta L, Bozzetta E, Goria M, Prearo M, Bercovier H. Enterococcus seriolicida is a junior synonym of Lactococcus garviae, a causative agent of septicemia and meningoencephalitis in fish. Curr Microbiol. 1996. 32:85–88.
15. Elliot JA, Collins MD, Piggott NE, Facklam RR. Differentiation of Lactococcus lactis and Lactococcus garvieae from humans by comparison of whole cell protein profile patterns. J Clin Microbiol. 1991. 29:2731–2734.
Article
16. Garvie EI, Bramley A. Streptococcus uberis: an approach to its classification. J Appl Bacteriol. 1979. 46:295–304.
17. Kitao T. Inglis V, Roberts RJ, Bromage NR, editors. Streptococcal infection. Bacterial Diseases of Fish. 1993. Oxford: Blackwell;196–210.
18. Kusuda R, Kawai K, Toyoshima T, Komatsu I. A new pathogenic bacterium belonging to genus Streptococcus isolated from an epizootic of cultured yellowtail. Bull Jpn Soc Sci Fish. 1976. 42:1345–1352.
19. Lee DC, Lee JI, Park CI, Park SI. The study on the causal agent of streptococcosis (Lactococcus garviae), isolated from cultured marine fish. J Fish Pathol. 2001. 14:71–80.
20. Mata AI, Gibello A, Casamayor A, Blanco MM, Dominguez L. Multiplex PCR assay for detection of Bacterial Pathogens associated with warm-water streptococcosis in fish. Appl Environ Microbiol. 2002. 68:5177–5180.
Article
21. Muzquiz JL, Royo FM, Ortega C, De Blas I, Ruiz I, Alonso JL. Pathogenicity of streptococcosis in rainbow trout (Orcorhynchus mykiss): dependence on age of diseased fish. Bull Eur Assoc Fish Pathol. 1999. 19:114–119.
22. Nieto JM, devesa S, Quiroga A, Toranzo AE. Pathology of Enterococcus sp. in farmed turbot, Scopthalmus maximus L. J Fish Dis. 1995. 18:21–30.
23. Pier GB, Madin SH. Streptococcus iniae sp. nov., a beta-hemolytic streptococcus isolated from an Amazon freshwater dolphin, Inia geoffrensis. Int J Syst Bacteriol. 1976. 26:545–553.
Article
24. Plumb JA. Major diseases of striped bass and redfish. Vet Hum Toxicol. 1991. 33:34–39.
25. Rasheed V, Plumb J. Pathogenicity of a non-hemolytic group B Streptococcus sp. in gulf killifish, Fundulus grandis (Baird and Girard). Aquaculture. 1984. 37:97–105.
Article
26. Riffon R, Sayasith K, Khalil H, Dubreuil P, Drolet M, Lagace J. Development of a rapid and sensitive test for identification of major pathogens in bovine mastitis by PCR. J Clin Microbiol. 2001. 39:2584–2589.
Article
27. Williams AM, Collins MD. Molecular taxonomic studies on Streptococcus uberis types I and II. Description of Streptococcus parauberis sp. nov. J Appl Bacteriol. 1990. 68:485–490.
Article
28. Williams AM, Collins MD. DNA fingerprinting of Streptococcus uberis based on polymorphism of DNA encoding rRNA. Lett Appl Microbiol. 1991. 12:23–28.
Article
29. Zlotkin A, Eldar A, Ghittino C, Bercovier H. Identification of Lactococcus garveiae by PCR. J Clin Microbiol. 1998. 36:983–985.
Full Text Links
  • JVS
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr