J Vet Sci.  2006 Mar;7(1):37-41. 10.4142/jvs.2006.7.1.37.

Analysis of Salmonella enterica serotype Enteritidis isolated from human and chickens by repetitive sequence-PCR fingerprinting, antibiotic resistance and plasmid profiles

• Suh DK
• Song JC

• KMID: 1103551
• DOI: http://doi.org/10.4142/jvs.2006.7.1.37

Abstract

A total of 22 Salmonella enterica serotype Enteritidis (S. Enteritidis) strains isolated from human and chicken were subjected to DNA fingerprinting by repetitive sequence PCR using ERIC and BOX primers, antibiotic resistance and plasmid patterns. Both ERIC and BOX PCR amplification data revealed a highly genetic homogeneity between isolates from human and chicken except one isolate, which originated from chicken and showed a different DNA band pattern from others. Eleven of 22 S. Enteritidis isolates (50%) were resistant to more than one antibiotics and characterized by 5 resistance patterns. The most common pattern was penicillin resistant (63.6%). Only one isolate from chicken showed a multiple drug resistance patterns to 4 antibiotics. All 22 S. Enteritidis isolates harbored more than two plasmids with eight different plasmid profiles including two to six plasmids with approximate molecular size ranging from 1.9 to 21 kb. A band of 15 kb size was detected in all isolates tested, however, the band sizes smaller than 15 kb were found only in isolates from chicken.

Keyword

antibiotic resistance; plasmid; rep-PCR fingerprinting; Salmonella enterica serotype Enteritidis

MeSH Terms

Animals
*Chickens
China/epidemiology
DNA Fingerprinting/veterinary
DNA, Bacterial/chemistry/genetics
Disease Outbreaks/*veterinary
Humans
Microbial Sensitivity Tests/veterinary
Microsatellite Repeats/genetics
Plasmids/chemistry/genetics
Polymerase Chain Reaction/veterinary
Poultry Diseases/epidemiology/*microbiology
Salmonella Food Poisoning/epidemiology/*microbiology
Salmonella enteritidis/drug effects/*genetics/isolation&purification

Figure

• 

  Fig. 1 DNA fingerprint patterns of S. Enteritidis strains from human (A) and chicken (B) by rep-PCR with the ERIC primers. (A) M, 100 bp ladder; Lane 1-12, SH01-SH12 in order. (B) M, 100 bp ladder; Lane 1-10, SC01-SC10 in order.

• 

  Fig. 2 DNA fingerprint patterns of 10 S. Enteritidis strains from chicken by rep-PCR with the BOX primer. M, 100 bp ladder; Lane 1-10, SC01-SC10 in order.

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