J Vet Sci.  2009 Jun;10(2):169-171. 10.4142/jvs.2009.10.2.169.

Two different types of malignant fibrous histiocytomas from pet dogs

• Do SH
• Hong IH
• Park JK
• Ji AR
• Kim TH
• Kwak DM
• Jeong KS

• KMID: 1102976
• DOI: http://doi.org/10.4142/jvs.2009.10.2.169

Abstract

We describe 2 cases of malignant fibrous histiocytomas (MFHs) that spontaneously developed in young pet dogs. To classify these tumors, we applied a panel of antibodies (vimentin, desmin, alpha-SMA, and ED1) and Azan staining for collagen. The MFHs were most consistent with osteoclast-like giant and inflammatory cell types. The first case had positive staining for ED1 and vimentin, and given the osteoclast-like giant cells, calcification sites accompanying peripheral giant cell infiltrates. The latter case, the inflammatory cell type, exhibited a storiform-pleomorphic variant of neoplastic cells, including an ossifying matrix. MFHs are among the most highly aggressive tumors occurring in soft tissue sarcomas in elderly dogs; however, MFHs have been poorly studied from a diagnostic point of view. Herein, we describe the histologic and immunohistologic features of MFHs in detail, thus classifying the subtypes of these tumors.

Keyword

dog; inflammatory; malignant fibrous histiocytoma; osteoclast-like giant cell

MeSH Terms

Animals
Biopsy/veterinary
Dog Diseases/diagnosis/*pathology
Dogs
Female
Histiocytoma, Malignant Fibrous/diagnosis/pathology/*veterinary
Immunohistochemistry/veterinary
Male
Soft Tissue Neoplasms/diagnosis/pathology/*veterinary

Figure

• 

  Fig. 1 Malignant fibrous histiocytoma (MFH), subtype osteoclast-like giant cell type (A-E). Osteoclast-like giant cells (arrow head) resembling normal osteoclast are scattered throughout the lesion, especially around the calcification sites. In addition to these features, peripheral giant cells (arrow) appeared (A). Immunostaining for vimentin, desmin, α-SMA, and ED1, respectively (B-E). Tumor cells were positive for vimentin (B), and negative for α-SMA (D) and ED1 (E). Note the strong positive for desmin of smooth muscle cells of blood vessels vs. negative staining of tumor cells (C). Osteoclast-like giant cells showed positive for ED1 (E). Inflammatory cell type of MFH (F-H). This type of MHF was characterized by infiltration of various inflammatory cells (F) and ossifying matrix (G). Note the bone matrix surrounding the neoplastic fibroblasts (H). A, F and G: H&E stain, B, C, D and E: ABC method counterstained with hematoxylin, H: Azan stain. A-E: ×66, F and G: ×132, H: ×33.

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