J Vet Sci.  2009 Jun;10(2):165-167. 10.4142/jvs.2009.10.2.165.

A novel beta-glucan produced by Paenibacillus polymyxa JB115 induces nitric oxide production in RAW264.7 macrophages

• Chang ZQ
• Lee JS
• Hwang MH
• Hong JH
• Jung HK
• Lee SP
• Park SC

• KMID: 1102975
• DOI: http://doi.org/10.4142/jvs.2009.10.2.165

Abstract

The effect of extracellular beta-(1-->3), (1-->6)-glucan, produced by Paenibacillus polymyxa JB115, on nitric oxide (NO) production in RAW264.7 macrophages was investigated. beta-glucan induced the production of NO by RAW264.7 macrophages in a concentration- and time-dependent manner. Moreover, beta-glucan stimulation increased the mRNA expression of iNOS, COX-2 and IL-6 in RAW264.7 macrophages in a concentration-dependent manner.

Keyword

beta-glucan; macrophages; nitric oxide; Paenibacillus polymyxa

MeSH Terms

Animals
Bacillus/*metabolism
Cell Line
Cyclooxygenase 2/biosynthesis/genetics
Interleukin-6/biosynthesis/genetics
Lipopolysaccharides/pharmacology
Macrophages/*drug effects/enzymology/immunology
Mice
Nitric Oxide/*biosynthesis/immunology
Nitric Oxide Synthase Type II/biosynthesis/genetics/metabolism
RNA, Messenger/biosynthesis/genetics
Reverse Transcriptase Polymerase Chain Reaction
beta-Glucans/metabolism/*pharmacology

Figure

• 

  Fig. 1 Effects of β-glucan and lipopolysaccharide (LPS) on the viability of RAW264.7 macrophages. Data represents the mean ± SD. *Significant difference (p < 0.05) compared to the control group.

• 

  Fig. 2 β-glucan induced nitric oxide production in RAW264.7 macrophages. RAW264.7 cells were treated with either LPS (0.5 µg/ml) or β-glucan. Data represents the mean ± SD. *Significant difference (p < 0.05) compared to the control group.

• 

  Fig. 3 β-glucan induced nitric oxide production in RAW264.7 macrophages. RAW264.7 cells were treated with β-glucan (300 µg/ml) for (0, 1, 2, 4, 6, 8, 12 or 24 h). Data represents the mean ± SD. *Significant difference (p < 0.05) compared to the control group.

• 

  Fig. 4 Role of polymyxin B (PB) on nitric oxide production in RAW264.7 macrophages treated with either LPS or β-glucan. RAW264.7 cells were pretreated with 50 µg/ml of PB for 30 min and then activated with either LPS (0.2 µg/ml) or β-glucan (300 µg/ml). Data represents the mean ± SD. *Significant difference (p < 0.05) compared to the control group, #Significant difference (p < 0.05) compared to the LPS group.

• 

  Fig. 5 β-glucan induced mRNA expression of cytokines in RAW264.7 macrophages. RAW264.7 cells were exposed to β-glucan at various concentrations, or LPS. After an 8 h incubation, i-NOS, COX-2, IL-6 and TNF-α mRNA were assessed by semi-quantitative RT-PCR.

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