J Vet Sci.  2008 Mar;9(1):109-111. 10.4142/jvs.2008.9.1.109.

Acute monoblastic leukemia in a FeLV-positive cat

• Prihirunkit K
• Narkkong N
• Apibal S

• KMID: 1102961
• DOI: http://doi.org/10.4142/jvs.2008.9.1.109

Abstract

A 1.6-year-old male domestic short hair cat was brought to the Veterinary Medical Teaching Hospital, Kasetsart University, with signs of severe anemia, depression, and general lymph node enlargement. Complete blood count revealed leukocytosis and massive undifferentiated blasts. Testing for antibodies specific to feline leukemia virus (FeLV) was positive, and FeLV nucleic acid was confirmed by nested polymerase chain reaction. Base on cytochemistry and ultrastructure, the cat was diagnosed with acute monoblastic leukemia.

Keyword

cat; cytochemistry; FeLV; monoblastic leukemia; ultrastructure

MeSH Terms

Animals
Cat Diseases/*diagnosis/*virology
Cats
Leukemia Virus, Feline/*isolation & purification
Leukemia, Monocytic, Acute/diagnosis/*veterinary/virology
Male
Osteoblasts/ultrastructure

Figure

• 

  Fig. 1 Morphologies and cytochemical stainings of undifferentiated blasts: (A and B) blasts were round to ovoid in shape with finely stippled nuclear chromatin and distinct nucleoli (Wright-Giemsa stain); (C) a blast with a prominent cytoplasmic tail (Wright-Giemsa stain); (D) a blast that stained positive for peroxidase; (E) a blast that was negatively stained for Sudan black B (left) compared to a positively-stained granulocyte (right); (F) a blast with positive Sudan black B staining; (G) a blast that was strongly positive for α-naphthyl acetate esterase; (H) a blast that was moderately positive for β-glucuronidase; (I) a blast that was positive for PAS.

• 

  Fig. 2 Cellular surfaces and ultrastructures of blasts: (A and B) Ruffled membrane with deep fissures and pseudopodic projection (SEM); (C) Indented nucleus with marginated chromatin and light cytoplasm with some electron dense granules and organelles (left) adhered to a plasma cell (right) (TEM); (D) High magnification of (C) showing the organelles and granules.

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