J Vet Sci.  2002 Mar;3(1):1-5.

Additive Estrogenic Activities of the Binary Mixtures of Four Estrogenic Chemicals in Recombinant Yeast Expressing Human Estrogen Receptor

Affiliations
  • 1Department of Veterinary Public Health, College of Veterinary Medicine, School of Agricultural Biotechnology, Seoul National University, Seodun-Dong, Kwonsun-Ku, Suwon 441-744, Korea. leeys@snu.ac.kr

Abstract

To evaluate the estrogenic activities of several chemicals such as 17 beta-estradiol (E2), rho-nonylphenol, bisphenol A, butylparaben, and combinations of these chemicals, we used recombinant yeasts containing the human estrogen receptor [Saccharomyces cerevisiae ER + LYS 8127]. We evaluated E2 was most active in the recombinant yeast assay, followed by rho-nonylphenol, bisphenol A, butylparaben. The combinations of some concentrations of 17-estradiol as a strong estrogen and bisphenol A or butylparaben as a weak estrogen showed additive estrogenic effects. Also, the combinations of some concentrations of nonlyphenol and butylparaben and combination of butylparaben and bisphenol A showed additive effects in the estrogenic activity. Therefore, the estrogenic activities of the combinations of two chemicals were additive, not synergistic.

Keyword

Recombinant yeast; human estrogen receptor; beta-galactosidase; combination; additive

MeSH Terms

Cloning, Molecular
Estradiol/pharmacology
Estrogens/classification/*pharmacology
Estrogens, Non-Steroidal/*pharmacology
Humans
Kinetics
Parabens/pharmacology
Phenols/pharmacology
Receptors, Estrogen/drug effects/*physiology
Recombinant Proteins/drug effects/metabolism
Saccharomyces cerevisiae/genetics
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