Korean J Ophthalmol.  2008 Jun;22(2):123-129. 10.3341/kjo.2008.22.2.123.

Short-term Efficacy of Topical Immunosuppressive Agents on the Survival of Cultivated Allo-Conjunctival Equivalents

Affiliations
  • 1Department of Ophthalmology, Dongguk University College of Medicine, Goyang, Korea.
  • 2Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea. kmk9@snu.ac.kr
  • 3Artificial Eye Center, Clinical Research institute Seoul National University Hospital, Seoul, Korea.
  • 4Valued eye clinic, Taegeon, Korea.
  • 5Woori eye clinic, Taegeon, Korea.
  • 6Korea Eye Center Hospital, Seoul, Korea.

Abstract

PURPOSE: To investigate the short-term efficacy of topical immunosuppressive agents on the survival of cultivated allo-conjunctival equivalents. METHODS: Twenty-five eyes of New Zealand white rabbits were included. Temporal conjunctivae were trephined to a diameter of 7.5 mm, and then cultured allo-conjunctival epithelial cells on amniotic membrane were transplanted onto them. Various immunosuppressants including steroid, cyclosporine, and rapamycin were applied topically four times a day for a week. Epithelial defects and graft edema were graded daily. Numbers of inflammatory cells were measured in H&E. PKH26 and cytokeratin 4 and 7 were immunostained. RESULTS: Earlier epithelialization was observed in 1% steroid-treated eyes and defects persisted significantly in 0.5% CsA applied eyes. In histology, PKH26 positive cells considered as donor cells were only found in 1% steroid or 0.01% rapamycin applied eyes. 1% steroid- or 0.01% rapamycin-applied eyes both showed positive staining for keratin-4 and -7. Inflammatory cells were less found in 1% steroid or 0.01% rapamycin treated eyes. CONCLUSIONS: Topical steroid or rapamycin can help to suppress acute inflammation and enhance the acute survival of transplanted conjunctival cells.

Keyword

Cultivated allo-conjunctival equivalents; Steroid; Topical immunosuppressants; Rapamycin
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