J Vet Sci.  2002 Dec;3(4):321-325.

Mutations of p53 Tumor Suppressor Gene in Spontaneous Canine Mammary Tumors

• Lee CH
• Kweon OK

Abstract

Mutation of the p53 tumor suppressor gene has been related in the pathogenesis of numerous human and canine cancers, including breast cancers and mammary tumors. We have investigated exons 5-8 of the p53 gene for mutations in 20 spontaneous canine mammary tumors using polymerase chain reaction (PCR) with direct sequence analysis to evaluate the role of this gene in canine mammary tumorigenesis and analyzed to compare with other clinicopathological parameters including age, histology, stage, recurrence and death from tumor. Four missense (one case had two missense mutations) and one nonsense mutations were detected in 10 malignant lesions (40%), and two missense and one silent mutations were found in 10 benign mammary tumors (30%). Five of the missense mutations were located in highly conserved domains II, III, IV and V. After a follow-up period, four dogs showed a progression and three of these patients revealed death from mammary carcinoma with p53 mutation. These results demonstrated that the p53 gene mutations might be involved in the development of canine mammary tumors and contribute to the prognostic status in canine mammary carcinomas.

Keyword

dog; mammary tumor; p53 gene mutation

MeSH Terms

Animals
Codon, Nonsense/genetics
DNA, Neoplasm/chemistry/genetics
Dog Diseases/*genetics
Dogs
Female
Genes, p53/*genetics
Mammary Neoplasms, Animal/*genetics
Mutation, Missense/genetics
Polymerase Chain Reaction/veterinary
Sequence Analysis, DNA
Survival Analysis
Tumor Suppressor Protein p53/genetics